安徽省知母药材质量研究

Quality Research on Anemarrhenae Rhizoma from Anhui Province

[目的]研究安徽产知母药材中知母皂苷BⅡ和芒果苷的含量.[方法]知母皂苷BⅡ采用Diamonsil C18柱（250 mm ×4.6 mm,5μm）、乙腈-水等度洗脱（25∶75）,流速1.0 ml/min、ELSD检测器漂移管温度60℃、载气流速3.0 ml/min、柱温为室温（25℃）;芒果苷采用Diamonsil C18柱（150 mm×4.6 mm,5μm）、乙腈-0.2％冰醋酸水溶液（15∶85）等度洗脱,流速1.0 ml/min.[结果]知母皂苷BⅡ在1.036～10.360 μg范围内（r=0.999 6）进样量的对数值与峰面积的对数值呈良好的线性关系,芒果苷在0.05 ～1.00 μg范围内（r=0.9994）进样量与峰面积呈良好的线性;知母皂苷BⅡ平均回收率为99.7％,RSD=1.19％;芒果苷平均回收率为100.3％,RSD=1.42％.[结论]安徽省知母药材中知母皂苷BⅡ与芒果苷含量均符合2010版药典含量标准.

[Objective] To study the quality of Anemarrhenae Rhizoma by determination of timosaponin BⅡand mangiferin content.[Method]（timosaponin BⅡ）The column used was a packed with 5μm stationary phase Diamonsil C18（250 mm ×4.6 mm）.The mobile phase consisted of acetonitrile-water （25∶75） at a flow rate of 1.0 ml/min.The temperature of drift tube was 60 ℃,and the nebulizer nitrogen flow rate was 3.0ml/min.（mangiferin）The chromatographic separation was achieved on a Diamonsil C18 column （150 mm ×4.6 mm,5 μm） with acetonitrile：0.2％ glacial acetic acid （15∶85） as mobile phases at the flow rate of 1.0 ml/min for isocratic elution.[Result] The linear range of the timosaponin BⅡ was 1.036-10.360 μg and the mangiferin was 0.05-1.00 μg ;The average recovery （n =6） of timosaponin BⅡand mangiferinin in crude material were 99.7 ％ and 100.3 ％ respectively with RSD of 1.19 ％ and 1.42％.[Conclusion] The contents of timosaponin BⅡ and mangiferin of Anemarrhenae Rhizoma from Anhui Province met 2010 Chp.Standards.