高效液相色谱-化学发光测定食品中的香兰素

Determination of vanillin by high performance liquid chromatography with chemiluminescence detection

在碱性条件下,香兰素对luminol-K3Fe（CN）6体系有增敏作用。根据这一现象建立了一种经高效液相色谱（HPLC）分离柱后测定香兰素的化学发光法。本方法以C18为色谱柱,用乙腈和水（50∶50,V/V）为流动相,线性范围7.0×10^-7～4.0×10^-5mol L-1,检出限为2.0×10^-7mol·L-1（3σ）,相对标准偏差为3.9%（c=4.0×10^-6mol/L,n=9）。该方法用于食品中香兰素含量的测定,其分析结果与高效液相-紫外光度法（HPLC-UV）比较具有很好的一致性。

In alkaline solution,vanitlin shows strong sensitivity enhancement effect to chemiluiminesecnce product by luminoI-K3Fe （CN）e system. According to this phenomenon, a kind of by high performance liquid chromatography （HPLC） coupled separation column after the determination of vanillin chemiluminescence method. This method was based on the C18 column,acetonitrile and water （50：50,V/V） as mobile phase,under the optimal conditions,the chemiluminescence intensity was correlated linearly with concentration of vanillin in the rang of 7.0× 10^-7N4.0×10^-5mol·L-1, and the determination limit was 2.0×10^-7mol· L-1 （3σ）, its relative standard deviation was 3.9% （c=4.0×10^4mol/L,n=9）. This method had been applied to the determination of vanillin in food samples,the analysis results agreed well those from HPLC-UV method.