培养条件对杜氏藻β-胡萝卜素含量的影响

Effects on culture conditions for β-carotene contents of Dunaliella sp.

为了优化杜氏藻的培养条件,对采自山西运城盐湖的杜氏藻（Dunaliella sp.）采用单因素和正交实验方法,研究了温度、盐度和氮、磷、铁元素对其细胞增殖和β-胡萝卜素积累的影响。结果显示杜氏藻的β-胡萝卜素含量与吸光度OD453nm之间存在良好的线性关系,利用吸光值表征杜氏藻β-胡萝卜素含量步骤简单,数据准确,适于高效快速对样品进行测定。杜氏藻在20～25℃,1.0～2.0mol/L Na Cl时,较有利于β-胡萝卜素积累。杜氏藻生长至对数期后,高浓度的氮源、磷源都不利于β-胡萝卜素的积累,而铁源在实验期间,对杜氏藻β-胡萝卜素的积累无影响。进一步优化,培养基中有利于β-胡萝卜素积累的Na NO3、KH2PO4和Fe C6H5O7的浓度最优组合为0.6200、0.0256、0.0010g/L。本实验得到了较适合杜氏藻积累β-胡萝卜素的培养基,为今后当地开发利用高产β-胡萝卜素藻株奠定了理论基础。

The single factor and orthogonal experiments were performed to optimize the cultivating conditions of Dunaliella sp. which was collected from salt lake in Yuncheng,Shanxi,China. Temperature,NaCI,N,P and Fe were studied for effects on β-carotene contents of Dunaliella sp. The results showed that there was a good linear relationship between β-carotene contents of Dunaliella sp. and OD4,β-The β-carotene contents of Dunaliella sp. was measured by the method of optical density which was simple,accurate and efficient. Dunaliella sp. was cultured under 20-25℃ and 1.0-2.0mol/L（NaCl） that were conducive to the β-carotene accumulation. When Dunaliella sp. cells grow to the logarithmic phase, high concentrations of N and P were not conducive to the accumulation of β-carotene,and the concentration of Fe had no effect on the accumulation of β-carotene. The optimum concentrations of NaNQ3,KH2PQ4 and FeCeH507 for β-carotene accumulation were 0.6200,0.0256,0.0010g/L. The medium for β-carotene accumulation of Dunaliella sp. was optimized for lay the theoretical foundation of future development and utilization of local high β-carotene production algae strains.