中性蛋白酶水解大麦虫蛋白工艺的研究

The hydrolysis of protein of Zophobas morio with neutrase

以大麦虫幼虫脱脂虫粉为原料,蛋白质水解率为评价指标,通过单因素和正交实验,确定中性蛋白酶水解大麦虫蛋白质的最佳工艺条件:酶解温度55℃,酶用量3.0%,固液比1∶5,酶解时间4h,p H为7.0。经过中性蛋白酶水解,蛋白质水解率达到10.05%。酶解后的蛋白酶解液呈深红棕色,澄清透明。

With non-fat zophobas morio larvae as raw material,the enzymolysising technology conditions were studied and the results evaluated by the hydrolysis rates of protein. Through single factor and orthogonal experiments,the optimum technological condition was confirmed：solid to water ratio was 1：5, dosage of enzyme 3.0%, pH7.0,temperature 55℃ to hydrolyze 4h. The experimental results were as follows =the degree of hydrolysis of Zophobas morio protain approached 10.05% and the solution was dark reddish brown,clear and transparent.