CEA和MUC-1mRNA在大肠癌中的表达及临床意义

Expression of CEA and MUC-1 m RNA in Colorectal Cancer and Its Clinical Significance

[目的]探讨大肠癌患者外周血中CEA、MUC-1 m RNA的表达及临床意义。[方法]应用巢式RT-PCR技术检测20名健康人、20例大肠腺瘤疾病患者和90例大肠癌患者外周血CEA m RNA和MUC-1 m RNA的表达。[结果]大肠癌患者CEA m RNA和MUC-1 m RNA表达的阳性率分别为53.3%(48/90)和52.2%(47/90);健康人外周血中无CEA m RNA,有12名MUC-1 m RNA表达,表达率为60.0%(12/20);大肠腺瘤疾病患者无CEA m RNA表达,有10例MUC-1 m RNA表达,表达率为50.0%(10/20)。大肠癌患者中CEA、MUC-1 m RNA表达与肿瘤分期、淋巴结转移有关(P<0.05)。[结论]CEA作为检测大肠癌患者外周血微转移的分子标志物具有良好的灵敏度和特异性,同时检测CEA和MUC-1可作为判断大肠癌病情进展的指标。

[Purpose] To investigate the expression of CEA m RNA,MUC-1 mRNA in colorectal cancer and its significance. [ Methods]Expression of CEA mRNA,MUC-1 mRNA was detected by nested RT-PCR in 20 healthy persons,20 cases with colorectal adenoma and 90 cases with colorectal cancer. [Results] The positive expression rate of CEA mRNA and MUC-1 mRNA was 53.3%（48/ 90） and 52.2%（47/90） in colorectal caner patients respectively. No CEA mRNA expression was found in healthy persons. The positive expression rate of MUC-I mRNA in healthy persons was 60.0%（12/20）. No CEA mRNA expression was found in 20 cases with colorectal adenoma diseases. The positive expression rate of MUC-I mRNA in colorectal adenoma patients was 50.0%. CEil mRNA,MUC-1 mRNA expression significantly correlated with tumor stage and lymph node metastasis （P〈0.05）. [Conclusion ] Molecular marker CEA mRN A as the detection for micrometas- tasis in peripheral blood of patients with coloreetal cancer has good sensitivity and specificity. Combined detection of CEA mRNA,MUC-1 mRNA can be used as markers to evaluate disease progression in patients with colorectal cancer.