ARTP诱变筛选草菇优良菌株及RAPD分析

Screening and RAPD Analysis of Volvariella volvacea ARTP Mutants

为构建草菇优良菌株突变体库,首次利用常压室温等离子体技术诱变草菇原生质体,优化的诱变条件是输出功率125 W、处理时间20 s。筛选出3株抗冻能力明显提高的突变菌株,3株突变株在草菇生长的20℃低温环境与对照相比生长速度提高了17%~57%,出菇爬料速度提高了0.8~2.4倍,出菇时间提前了1~2d,抗低温胁迫能力提高了24h。研究运用RAPD技术进行分子鉴定,建立草菇ARTP诱变育种筛选与检测评价平台。

In this study,it was the first time for ARTP to apply in Volvariella volvacea breeding by protoplast mutation,for the construction of an excellent mutants library of Volvariella Volvacea. The optimum conditions of V. volvacea strain mutation were determined,which were125 W as output power and 20 s as mutagenesis time. After screening,three mutant strains with stronger low-temperature resistant were selected by superior physiological trait,of which the mycelia growth speed increased by 17%- 57% under 20 ℃,the overgrowth speed of mycelium on culture medium increased by0. 8- 2. 4 times and maturation time were shorten for 1- 2 days. By RAPD analysis,three strains were molecular identified,showing clearly different from the parental strain. The results herein demonstrate that the technology platform was built for ARTP mutation breed,detection and evolution for V. volvacea.