不同性别人群的蛋白质N-糖基结构谱型比较

Comparison of structure profiles of protein N-glycosylation between two genders

目的对不同性别人群的血浆N-糖基结构谱型进行分析比较,为基础人群N-糖基结构的分布提供科学依据。方法采用整群抽样,以2009年4~7月在北京某社区调查的212名居民为研究对象,基于高效液相色谱法进行血浆糖基组检测。不同性别的糖基含量比较采用两独立样本t检验或非参检验,P〈0.05为差异有统计学意义。结果 212名研究对象的平均年龄为（37.78±17.89）岁,性别比为0.88。半乳糖基化修饰水平,男性双触角非半乳糖聚糖水平略高（Z=2.33,P=0.02）,女性单半乳糖聚糖水平较高（t=-2.06,P=0.04）。岩藻糖基化分析显示,女性核心岩藻糖化聚糖水平略高（t=-2.16,P=0.03）。唾液酸糖基化水平中,女性单唾液酸糖基化水平较高（t=-2.43,P=0.02）,男性二唾液酸糖基化水平较高（t=2.11,P=0.04）。结论血浆N-糖基结构水平可能存在性别决定性。

Objective To analyze and compare the structure profiles of plasma N-glycosylation between two genders in order to provide scientific basis for the distribution of N-glycosylation. Methods A cluster sampling method was used to randomly select 212 residents from a community in Beijing.Blood samples were collected and plasma N-glycans were identified by high performance liquid chromatography（HPLC）. ResultsThe average age of subjects was 37.78±17.89 years with the sex ratio of 0.88.The levels of galactosylation,fucosylation and Sialic glycosylation of N-glycans were all significantly different between male and female subjects（P〈0.05）,with more monogalactosylated glycans,core-fucosylated glycans and monosialo in women,and more biantennary non-galactosylated glycans and disialo in men. Conclusions The plasma N-glycome profile is gender dependent,therefore,it is very important to consider this difference when the glycome markers are to be developed.