不同来源副溶血性弧菌血清群耐热直接溶血素及耐药性研究

Studies on the serotypes,thermostable direct hemolysin and drug resistance of Vibrio parahaemolyticus

目的了解不同来源副溶血性弧菌血清群分布,耐热直接溶血素携带情况及耐药性状况。方法利用血清凝集试验进行血清群检测;利用荧光定量PCR仪进行耐热直接溶血素检测;采用纸片扩散法进行11种抗生素药物敏感试验;采用χ2检验进行统计学分析。结果副溶血性弧菌食物中毒和肠道门诊分离株血清群以O3和O4为主,水产品分离株无优势血清群。副溶血性弧菌食物中毒和肠道门诊分离株耐热直接溶血素检出率均达90%以上,经χ2检验差异无统计学意义(χ2=0.03,ν=1,P=0.86)。副溶血性弧菌水产品分离株耐热直接溶血素检出率为0。62株副溶血性弧菌对氯霉素、四环素和复方磺胺甲噁唑100%敏感。结论副溶血性弧菌食物中毒和肠道门诊分离株与水产品分离株的血清群和耐热直接溶血素存在较大差异。抗生素首选氯霉素、四环素和复方磺胺甲噁唑。

Objective To analyze the serotypes,thermostable direct hemolysin and drug resistance of Vibrio parahaemolyticus isolated in Jianggan. Methods Serotypes of Vibrio parahaemolyticus were determined by serum agglutination test.Real-time PCR was used for the detection of thermostable direct hemolysin,and drug resistance test for 11 antibiotics was performed by disc diffusion method.Chi-square test was applied for data statistical analysis. Results Serotype 03 and 04were found to be dominant among strains isolated from samples of food poisoning and gastrointestinal outpatient,while there was no dominant serotype found in aquatic products.The detection rates of thermostable direct hemolysin from samples of food poisoning and other gastrointestinal outpatient were all over 90% without statistical difference（χ2=0.03,ν=1,P=0.86）.No thermostable direct hemolysin was detected in bacteria strains isolated from aquatic products.62 strains were 100%sensitive to chloramphenicol,tetracycline and SMZ-TMP. Conclusions Serotypes and thermostable direct hemolysin are different among Vibrio parahaemolyticus strains isolated from food poisoning,other gastrointestinal outpatients and aquatic products.Ideal antibiotics for treatment of vibrio parahaemolyticus are chloramphenicol,tetracycline and SMZ-TMP.