抗耶氏肺孢子菌合成抗原IgG和IgM及IgA抗体水平调查

Survey of antibodies to Pneumocystis jirovecii recombinant antigen

目的利用北京地区随机人群,抗耶氏肺孢子菌（Pneumocystis jirovecii,Pj）人工合成主要表面糖蛋白（Re-combinant major surface glycoprotein）,调查抗Pjr Msg、Ig G、Ig M和Ig A抗体水平,探讨人群感染Pj情况。方法利用原核表达的Pj r Msg可溶抗原,建立酶联免疫吸附试验,调查266例北京地区随机人群血清抗Pj r Msg Ig G、Ig M和Ig A抗体的检出情况。结果北京地区266例随机血清,抗Pj r Msg Ig G、Ig M和Ig A抗体检出率分别为42.5%（113/266）,41.7%（111/266）和0（0/266）。其中同期Ig G阳性Ig M阳性、Ig G阳性Ig M阴性、Ig G阴性Ig M阳性和Ig G阴性Ig M阴性的检出率分别为17.3%（46/266）、25.2%（67/266）、24.4%（65/266）和33.1%（88/266）。40-50岁组的抗Pj r Msg Ig M抗体检出率为62.8%（59/94）,分别高于20-29岁组的32.9%（28/85）（χ2=15.895,P=0.000）和30-39岁组的27.6%（24/87）（χ2=22.522,P=0.000）。结论北京地区成人抗Pj r Msg Ig G与Ig M抗体阳性率分别为42.5%和41.7%,人群感染Pj后的Ig G或Ig M抗体阳性率高达66.9%,Ig A抗体没有检出以及Ig M抗体在高年龄组中检出率高的原因需要进一步研究。

Objective To obtain recombinant major surface glycoprotein（rMsg）of Pneumocystisjiroveeii（Pj） and surveythe levels of anti-Pj rMsg IgG, IgM and IgA antibodies by using enzyme-linked immunosorbent assay（ELISA） among randomlyselected serums from Beijing local population. Methods ELISA method was established to detect anti-Pj rMsg IgG, IgM andlgA antibodies. The 266 serum samples randomly selected from Beijing Friendship Hospital were screened. Results Thepositive rate of anti-Pj rMsg IgG, IgM, and IgA antibodies were 42.5% （113/266） , 41.7% （111/266） , and 0 （0/266）respectively. The detection rates of simultaneously IgG positive IgM positive, IgG positive IgM negative, IgG negative IgMpositive and IgG negative IgM negative were 17.3% （46/266） , 25.2% （67/266） , 24.4% （65/266） and 33.1% （88/266）respectively. The positive rate of anti-Pj rMsg IgM antibody in （40-50） ages group was 62.8% （59/94） higher than that in （20-29） ages group （32.9%,28/85） （X2=15.895, P=0.000） or that in （30-39） ages group（ 27.6% ,24/87） （X2 = 22.522, P=0.000）.Conclusion The positive rates of anti-Pj rMsg IgG and IgM antibodies were 42.5% and 41.7% in Beijing adult population.The serological positive rate in population either anti-Pj IgG or IgM antibody positive after Pj infection was as high as 66.9%.That the anti-rMsg IgM positive rate was higher in elder ages group and anti-rMsg IgA positive rate was zero need furtherstudies.