氮源与其补加方式对1,3-丙二醇生物合成的影响

The Effect of Nitrogen and Its Adding Modes on 1,3-Propanediol Biosynthesis

氮源是发酵法生产1,3-丙二醇的一种重要原料,培养基中氮源浓度的高低直接影响了发酵结果.从氮源的选择与补加方式着手,研究了各种有机氮与无机氮的使用及补加方式对发酵的影响.研究表明:纯有机氮源无法满足1,3-丙二醇生产的需求,而3.0 g/L的玉米浆粉可以有效替代1.0 g/L的酵母浸粉成为工业大生产中的有机氮源,并且采用与p H值控制耦合的方式进行补加,使甘油转化生产1,3-丙二醇的最终含量提高到72.5 g/L.

Nitrogen source is an important raw material trogen concentration in the medium directly influences for the production of 1,3-propanediol fermentation, ni- the fermentation results, from the choice of nitrogen source and adding mode, research the use of a variety of organic nitrogen and inorganic nitrogen, the effect of addition on fermentation. Through the study, the pure organic nitrogen sources can not appease the demand of PDO production, while the corn steep powder 3.0 g/L as organic nitrogen source can be used in industrial production effectively instead of yeast extract. With the compelement model of uniting the PH value control model, the final content of 1,3-propylene glycol glycerol was increased to 72.5 g/L.