As_2O_3磁性Fe_3O_4白蛋白微球的制备与表征

Preparation and characterization of As_2O_3-loaded magnetic Fe_3O_4 albumin nanospheres

目的 ：制备用于肿瘤热化疗和逆转多药耐药的As2O3磁性Fe3O4白蛋白微球并表征。方法：化学共沉淀法制备Fe3O4磁性纳米粒,运用透射电镜、X射线衍射分析进行表征,溶血实验及MTT试验进行毒理学评定。去溶剂化交联法制备As2O3磁性Fe3O4白蛋白微球,运用透射电镜和能谱仪进行表征,在交变磁场作用下进行体外升温试验,体外释药方法研究其释药速率。结果：Fe3O4磁性纳米粒近似球形,粒径约20 nm,无溶血作用,细胞毒性为1级。As2O3磁性Fe3O4白蛋白微球近似球形,大小均匀,粒径约193.4 nm,其不同浓度的磁流体在交变磁场下可升温至39.5～58.0℃并保持恒定。体外释药实验证实As2O3磁性Fe3O4白蛋白微球具有明显的缓释功能。结论：Fe3O4磁性纳米粒作为药物载体具有良好的生物相容性。用去溶剂化交联法可以成功制备出As2O3磁性Fe3O4白蛋白微球,As2O3磁性Fe3O4白蛋白微球释药速率缓慢,为研究肿瘤热化疗和逆转多药耐药提供理论和实验基础。

Objective：To study the preparation and characterization of As2O3-loaded magnetic Fe3O4 albumin nanospheres which could be used in thermochemotherapy and reversing multidrug resistance of tumor. Methods：Fe3O4magnetic nanoparticles were prepared by chemical co-precipitation method. Their characteristics were observed by transmission electron microscope（TEM）,X-ray diffraction（XRD）,and their cytotoxicity was measured by hemolytic test and MTT assay. As2O3-loaded magnetic Fe3O4 albumin nanospheres were prepared by desolvation-crosslinking method. Their characteristics were observed by TEM and energy dispersive spectrometer（EDS）. Their hyperthermia induced by alternating magnetic field（AMF） was explored in vitro. The release rate of AS2O3 from the nanospheres was assessed by a dissolution test in vitro. Results：The prepared Fe3O4 magnetic nanoparticles were approximately spherical. The diameter was about 20 nm. They had no hemolysis activity and their cytotoxity was 1 class. The prepared As2O3-loaded magnetic Fe3O4 albumin nanospheres were approximately spherical,uniform in size observed by TEM. The diameter was about 193.4 nm. The temperature of magnetic fluid with different concentrations could rise to 39.5-58.0℃,and the magnetie fluid keep stable in AMF. The drug release test in vitro showed that As2O3-loaded magnetic Fe3O4 albumin nanospheres had a slow release rate.Conclusion：As a drug carrier,magnetic Fe3O4 nanoparticle is a kind of good biocompatibility material. The As2O3-loaded magnetic Fe3O4 albumin nanospheres can be prepared by desolvation-crosslinking method. They have an obvious effect of slow release of drug which may provide a theoretical and practical basis for further study of tumor thermochemotherapy and reversing multidrug resistance.