摘要
采用气相色谱法（GC）分析姬松茸中脂肪酸组成,样品用氢氧化钾-甲醇法甲酯化,正己烷定容,氢火焰离子检测器（FID）检测,并用9种脂肪酸混标定性,归一化法计算含量。结果显示姬松茸中脂肪酸主要有十二烷酸（月桂酸）、十四烷酸（肉豆蔻酸）、十六烷酸（棕榈酸）、十八烷酸（硬脂酸）、十八碳烯酸（油酸）、十八碳二烯酸（亚油酸）、十八碳三烯酸（亚麻酸）、二十烷酸（花生酸）等8种脂肪酸,其中亚油酸含量最高,不饱和脂肪酸占总量的79.493%。该方法测定姬松茸中脂肪酸成分,方便、快速、准确。
The chemical composition and contents of fatty acid in Agaricus blazei was determined by GC.After methylesterifi ed,The samples were analysed by the FID.The result showed containing several kinds of fatty acid,such as：Lauric acid,Myristic acid,palmitic acid,Stearic acid,oleic acid,Linoleic acid,Linolenic acid and Arachidic acid.The highest fatty acid was Linoleic acid in Agaricus blazei,and the polyunsaturated fatty acid was 79.493%.This mothod is a simple and quick method to determine fatty acids in Agaricus blazei Murill.
出处
《现代科学仪器》
2014年第4期137-139,共3页
Modern Scientific Instruments
关键词
姬松茸
脂肪酸
气相色谱
GC
Agaricus blazeik
Fatty acid
gas chromatography
GC
