江西省食源性沙门菌血清分型及脉冲场凝胶电泳指纹图谱研究

Serotyping and PFGE type of Salmonella isolates from food in Jiangxi Province

目的研究江西省食源性沙门菌的血清型,用脉冲场凝胶电泳(PFGE)方法对优势血清型进行分子分型,建立江西省食源性沙门菌PFGE指纹图谱资料库,为食源性疾病溯源提供数据。方法对136株食源性沙门菌进行血清分型,对其中优势血清型德尔卑沙门菌菌株进行PFGE分型,获得分子分型指纹图谱,运用Bionumerics v6.6软件进行聚类分析。结果 136株沙门菌分离株血清群以B群为主,优势血清型主要是德尔卑沙门菌(49.26%),67株德尔卑沙门菌可分为41个型别的PFGE指纹图谱(相似值100%的为同一PFGE型别),主要集中在3个型别中。结论江西省食源性沙门菌血清型分布呈多样性,优势血清型是德尔卑沙门菌,生肉制品是其主要的来源,同种血清型别的PFGE指纹图谱无相关性。

Objective To study the serotypes and molecular characteristics of foodborne Salmonella in Jiangxi Province by pulsed field gel electrophoresis（ PFGE）,and establish the PFGE fingerprint data bank. Methods A total of 136 foodborne Salmonella strains were identified by biochemical tests and serotyping. The S. derby were further typed by PFGE and established the fingerprint data bank,and clustering analysis was conducted by Bionumerics v6. 6. Results Among136 Salmonella isolated,the main serogroup were group B,and the dominant serotypes were S. derby（ 49. 26%）. 67 S. derby strains were 41 PFGE fingerprint types,3 PFGE fingerprint types were predominant in the PFGE fingerprint types.Conclusion Serotyping of foodborne Salmonella were phenotypically diverse. The S. derby were predominant. Raw meat products were the main source of foodborne Salmonella. PFGE fingerprint types were not related with serotypes.