丙酮酸激酶M2亚型在肾透明细胞癌组织中的表达及临床意义

The expression and significance of pyruvate kinase type M2 in clear cell renal cell carcinoma

目的 探讨丙酮酸激酶M2亚型（PKM2）在肾透明细胞癌组织及癌旁组织中的表达及临床意义.方法 收集48例肾透明细胞癌组织及其配对的癌旁组织,采用Western blot和实时荧光定量聚合酶链反应（FQ-PCR）及免疫组织化学的方法检测PKM2在肾透明细胞癌组织和配对癌旁组织中的表达水平,并分析其表达水平与肾透明细胞癌临床病理特征的关系.结果 Westernblot和FQ-PCR结果提示：肾透明细胞癌组织中PKM2蛋白的相对表达量为1.585±0.112,癌旁组织的相对表达量为0.760±0.097.肾透明细胞癌组织中PKM2 mRNA的相对表达量为5.060±0.471,癌旁组织的相对表达量为3.431±0.221.免疫组织化学提示：PKM2的阳性染色颗粒主要位于细胞质内,PKM2在28例肾透明细胞癌组织中高表达（58.33％）,在癌旁组织中仅5例高表达（10.41％）,提示PKM2在肾透明细胞癌组织的表达强度明显高于癌旁组织,差异均有统计学意义（P＜0.05）.PKM2在肾透明细胞癌组织中的表达,随Fuhrman分级和TNM分期的增高,有升高趋势.结论 PKM2在肾透明细胞癌组织中高表达,其表达随病理分级和临床分级的的升高呈升高趋势,PKM2可能在肾透明细胞癌的发生、发展中起重要作用.

Objective To investigate the expression of pyruvate kinase type M2 （PKM2） in human clear cell renal cell carcinoma tissues with regard to related clinicopathologic significance.Methods 48 cases of clear cell renal cell carcinoma tissues and matched normal adjacent tissues were collected.The expression of PKM2 was detected by immunohistochemistry,Western blotting and real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）.The relationship between clinicopathologic parameters and PKM2 expression was analyzed.Results The relative expression of PKM2 protein and mRNA in Clear cell renal cell carcinoma tissues are 1.585 ± 0.112 and 5.060 ± 0.471 by Western blotting and FQ-PCR respectively.And the relative expression of PKM2 protein and mRNA in matched adjacent normal kidney tissues are 0.760 ±0.097 and 3.431-±0.221.By immunohistochemistry,PKM2 protein mainly stained in the cytoplasm.High expression of PKM2 were noticed in 28 clear cell renal cell carcinoma tissues,accounting for 58.33%.In adjacent normal kidney tissues,only 5 cases showed high expression,accounting for 10.41%.And the differences are statistically significant.Higher PKM2 expression was associated with higher clinical stage and pathological grade.Conclusion PKM2 protein and mRNA have a significantly high expression in clear cell renal cell carcinoma tissues.PKM2 expression associated with tumor grade and pathological stage.PKM2 may be involved in the occurrence and progression of clear cell renal cell carcinoma.