血管抑素基因治疗Lewis肺癌移植瘤小鼠的疗效研究

Curative effectiveness of angiostatin gene therapy for lewis lung carcinoma xenografts in mice

目的 探讨血管抑素基因（PAng）治疗Lewis肺癌移植瘤小鼠的效果.方法 45只Lewis肺癌移植瘤模型的小鼠,根据对肺癌移植瘤小鼠治疗方式的不同将其随机分为3组,每组小鼠15只,分别为A组（空白组）、B组（空白脂质体组）以及C组（PAng组）,观察治疗前后肿瘤体积、重量的变化以及比较肺表面转移灶数目和小鼠的平均生存周期.结果 PAng组小鼠治疗21 d后的移植肿瘤体积为（189 ± 20） mm3,与A组（5 324±246） mm3、B组（5 367 ±326） mm3比较,差异均有统计学意义（P＜0.05）;PAng组小鼠治疗21 d后的移植肿瘤重量为（3.54 ±0.48）g,与A组（4.21 ±0.58）g、B组（4.26 ±0.59）g比较,差异均有统计学意义（P＜0.05）;PAng组小鼠治疗后的肺表面转移灶数目为（4.30 ±0.42）个、平均生存周期为（54.6±6.52）d,与对照组比较差异有统计学意义（P＜0.05）.结论 采用PAng的治疗方式具有比较理想的治疗效果.

Objective To investigate the effect of angiostatin gene therapy in mice with Lewis lung cancer xenografts.Methods Forty-five mice of Lewis lung carcinoma xenograft models were randomly divided into three groups （n =15 each）：group A （saline control group）,group B （blank liposome group）and group C （PAng group）.Tumor volume,weight change,the number of metastases on the lung surface,and the average survival of mcie were observed and compared.Results Volume of mouse transplanted tumor in PAng group [（189 ±20） mm3] was significantly lower than the control rats [A group：（5 324 ±246） mm3] ; B group：（5 367 ±326） mm3 （P 〈0.05）.Weight of mouse transplanted tumor in PAng group [（3.54 ±0.48） g] was significantly lower than A group [（4.21 ±0.58） g] and B group [（4.26 ±0.59） g,P 〈 0.05].Number of pulmonary metastatic foci after PAng treatment （4.30 ± 0.42） was much fewer compared with the control rats （P 〈 0.05） ; Average survival period of mice in PAng group [（54.6 ±6.52） d] was longer than control group （P 〈 0.05）.Conclusion The use of antiangiogenic gene therapy approach has the ideal therapeutic effect.