老鹳草素对成骨细胞增殖和骨保护素表达的影响

Effects of geraniin on osteoblastic proliferation and osteoprotegerin expression

目的 观察老鹳草素对成骨细胞增殖和骨保护素（OPG）表达的影响.方法 培养小鼠成骨细胞MC3T3-E1,噻唑蓝（MTT）法观测老鹳草素对成骨细胞增殖能力的影响.免疫细胞化学染色法和原位杂交技术检测老鹳草素对成骨细胞OPG蛋白和mRNA表达的影响.结果 MTT法测得对照组成骨细胞吸光度值为0.777±0.088、10-12 ～ 10-8 mol/L老鹳草素组分别为0.958±0.054、0.943±0.029、0.918±0.070、0.899±0.048和0.793±0.074;与对照组比较,除10-8 mol/L组外,差异有统计学意义（P＜0.01）.对照组和10-11～10-8 mol/L老鹳草素组OPG蛋白平均吸光度值分别为0.231±0.022、0.249±0.015、0.255±0.021、0.302±0.025和0.328±0.021;OPG mRNA平均吸光度值分别为0.297 ±0.041、0.307±0.039、0.318 ±0.035、0.394±0.036和0.429±0.050,提示老鹳草素呈浓度依赖性上调成骨细胞OPG表达.结论 老鹳草素具有促进成骨细胞增殖的作用,OPG表达上调可能是其中的机制之一.

Objective To observe the effects of geraniin on osteoblastic proliferation and osteoprotegerin （OPG） expression.Methods The osteoblasts （MC3T3-E1） were cultured with different concentrations of geraniin.Cell proliferation was assessed by methyl thiazolyl tetrazolium （MTT） colorimetric assay.The protein and mRNA expression levels of OPG were measured by immunocytochemistry and in situ hybridization （ISH） respectively.Results The absorbance values of the control group and geraniin （10-12-10-8mol/L） groups were 0.777 ±0.088,0.958 ±0.054,0.943 ±0.029,0.918 ±0.070,0.899 ± 0.04 and 0.793 ± 0.074 respectively with the differences being statistically significant between control group and geraniin groups except 10-8 mol/L geraniin group.The average absorbance values of OPG protein in the control group and geraniin （10-12-10-8 mol/L） groups were 0.231 ± 0.022,0.249 ± 0.015,0.255 ±0.021,0.302 ± 0.025 and 0.328 ± 0.021 respectively; and those of OPG mRNA were 0.297 ±0.041,0.307 ±0.039,0.318 ±0.035,0.394 ±0.036 and 0.429 ±0.050 respectively,showing geraniin promoted the protein and mRNA expression of OPG in a concentration-dependent manner.Conclusion It suggests that geraniin promotes osteoblast proliferation and upregulates the protein and mRNA expression of OPG.