染色体1p/19q杂合性缺失与少突胶质细胞肿瘤临床病理特征的关系

Relationship between the loss of heterozygosity on chromosome 1p/19q and clinicopathologic feature in oligodendroglial tumors

目的 探讨染色体1p/19q杂合性缺失与少突胶质细胞肿瘤临床病理特征的关系.方法 采用荧光免疫原位杂交技术检测73例少突胶质细胞肿瘤1p/19q杂合性缺失,并随访观察预后.结果 少突胶质细胞肿瘤年轻患者（＜ 60岁）1p/19q缺失高于老年患者（＞60岁,P＜0.01）;好发部位（额、顶、枕叶）的少突胶质细胞肿瘤1p/19q缺失与其他部位比较差异无统计学意义（P＞0.05）,与性别及肿瘤级别均无明显相关（P＞0.05）;少突胶质细胞肿瘤患者存在1p/19q联合缺失的中位生存时间为74个月,5年总生存率为72％;仅存在1p缺失者中位生存时间为43个月,5年总生存率为29％;而不存在1p/19q缺失者中位生存时间39个月,5年总生存率为18％.1p/19q联合缺失者中位生存时间长于仅存在1p缺失组,差异有统计学意义（P＜0.05）;且1p/19q缺失组中位生存时间也长于1 p/19q未缺失组,差异有统计学意义（P＜0.05）.仅存在1p缺失组中位生存时间虽然长于不存在1p/19q缺失组,但差异无统计学意义（Pp＞0.05）.结论 少突胶质细胞肿瘤1p/19q缺失与患者年龄明显相关,与患者性别、肿瘤发生部位及肿瘤级别无明显相关;少突胶质细胞肿瘤中染色体1p/19q杂合性缺失对患者的化疗敏感性及预后有关,可以作为分子分型的标准.

Objective To investigate the relationship between the loss of heterozygosity （LOH）on chromosome 1p/19q and clinicopathologic feature in oligodendroglial tumors.Methods The LOH on chromosome 1 p/19q was detected with fluorescence in situ hybridization in 73 patients with oligodendroglial tumors,and the prognosis was followed up.Results The positive rate of the LOH on chromosome 1p/19q in oligodendroglioma in younger patients （〈 60 years old） was higher than in older patients （〉 60 years old）,（P 〈 0.01）.There was no statistically significant difference in the LOH on chromosome 1 p/19q betwcen the oligodendroglial tumors at frontal lobe and other lobes （P 〉 0.05）.There was no statistically significant difference in the LOH on chromosome 1p/19q in oligodendroglioma between low grade and high grade （P 〉0.05）.There was no statistically significant difference in the LOH on chromosome 1p/19q in oligodendroglioma between males and females.The median survival time of patients with the LOH on chromosome 1 p/19q of oligodendroglioma was 74 months,and total 5-year survival rate was 72%.The median survival time in patients with 1 p LOH was 43 months,and the total 5-year survival rate was 29%.The median survival time of patients without 1 p/19q LOH was 39 months,and total 5-year survival rate was 18%.The median survival time of patients with 1p/19q LOH was significantly longer than that of those with 1 p LOH （P 〈 0.05）.The median survival time of patients with 1 p/19q LOH was significantly longer than that of those without 1 p/19q LOH （P 〈 0.05）.The median survival time of patients with 1 p LOH was longer than that in those without 1p/19q LOH,but there was no statistically significant difference （P〉0.05）.Conclusion The LOH on chromosome 1 p/19q in oligodendroglioma was obviously correlated with patients＆#39; age,but not with gender,tumor location and tumor grade.There were correlations between the LOH on chromosome 1 p/19q of oligodendroglioma and chemotherapy sensibility and prognosis.The LOH on chromosome 1p/19q of oligodendroglioma can act as the classified standard of molecular subtyping.