乙型肝炎病毒基本核心启动子区热点突变与广西肝癌家族聚集性的相关性

Relationship between hotspot mutations in basic core promoter of hepatitis B virus and familial clustering of hepatocellular carcinoma in Guangxi

目的:探讨乙型肝炎病毒(HBV)基本核心启动子(BCP)区突变与广西肝癌家族聚集的相关性。方法:从广西肝癌高发及无癌家族中配对选取HBs Ag阳性成员各103例。采用聚合酶链反应(PCR)扩增BCP区并测序分析。结果:BCP区突变发生在前5位的热点位点为T1762、A1764、G1775、V1753、G1803。单因素分析:HBV DNA≥105copies/m L、T1762、A1764和V1753突变均与肝癌高发有关(P<0.05)。多因素Logistic分析显示:HBV DNA≥105copies/m L和A1764突变是肝癌高发的独立危险因素。结论:HBV DNA水平、BCP区突变与广西肝癌家族聚集存在相关性。

Objective To explore the relationship between mutations in basic core promoter （BCP） of hepatitis B virus （HBV） and familial clustering of hepatocellular carcinoma （HCC） in Guangxi. Methods 153 pairs of members with HBsAg-positive were selected and matched from HCC high-incidence families and carcinoma-free families in Guangxi. The BCP genes were amplified and sequenced. Results The hotspot sites of the previous five mutations in BCP were T1762, A1764, G1775, V1753, G1803. In univariant analysis, HBV DNA≥10^5 copies/mL, T1762, A1764 and V1753 mutations were associated with the HCC high-incidence （P 〈0.05）. The multivariate logistic analysis showed that HBV DNA≥10^5 copies/mL and A1764 were independent risk factors for it. Conclusion HBV DNA level, the mutations in BCP showed correlations with familial clustering of HCC in Guangxi.