4种蛋白质／多肽在结核病患者与健康人血清中的表达和意义

Expression and significance of four kinds of serum protein / polypeptide in tuberculosis patients and healthy controls

目的:评价质荷比(m/z)分别为1 060、1 944、2 081和3 954的4种血清蛋白质/多肽(以下分别用A、B、C和D表示)区分结核病患者与健康人的能力。方法:应用表面增强激光解析-电离飞行时间质谱技术检测57例结核病患者和30例健康人的血清蛋白质谱,分析其A、B、C、D中的表达水平(用相对强度表示)并比较其差异,并应用诊断性试验评价方法分析其区分结核病患者群与健康人群的能力。结果:(1)A、B、C、D在结核病患者和健康人中的表达水平分别为1±11、1 597±3 102、460±765、1 208±1 003和123±201、47±98、36±93、397±355,两者差异显著。(2)以A、B、C、D血清表达水平为指标判定结核病患者和健康人的ROC曲线下面积分别为0.644、0.848、0.735和0.810,分界值分别为≤166、≥318、≥48和≥728。结论:B、C和D 3种血清蛋白质/多肽具有较好的区分结核病患者与健康人的能力,有望作为新型结核病诊断候选标志物。

Objective To assess the efficacy of using four kinds of proteins / peptides to distinguish the tuberculosis patients from healthy people. Methods A, B, C and D were used to represent four proteins /peptides with 1 060, 1 944, 2 081 and 3 954 of mass to charge ratio （m / z） in serum, respectively. Levels A, B, C and D in serum of 57 patients with tuberculosis and 30 healthy people were determined by using the surface-enhanced laser desorption-ionization time of flight mass spectrometry （SELDI-TOF-MS）. Then the differences of levels of f A, B, C and D were anlyzed between tuberculosis patients and healthy people. The efficacy of distinguishing tuberculosis patient from healthy people were evaluated by using diagnostic test evaluation method. Results （1） The levels of A, B, C and D were 1 ± 11, 1 597 ± 3 102, 460 ± 765 and 1 208 ± 1 003 in tuberculosis patients, while they were 123 ± 201, 47 ± 98, 36 ± 93 and 397 ± 355 in healthy people. （2） The area under the receiver operator characteristic （ROC） curve was 0.644, 0.848, 0.735 and 0.810 respectively. The serum levels of A, B, C and D could be used to distinguish tuberculosis patient from healthy people and the cut-off values of A, B, C and D were ≤166, ≥318, ≥48 and ≥728, respectively. Conclusions B, C and D have better performances to distinguish tuberculosis patients from healthy people , which may be regarded as new promising candidate markers for diagnosis of tuberculosis.