期刊文献+

4种蛋白质/多肽在结核病患者与健康人血清中的表达和意义

Expression and significance of four kinds of serum protein / polypeptide in tuberculosis patients and healthy controls
下载PDF
导出
摘要 目的:评价质荷比(m/z)分别为1 060、1 944、2 081和3 954的4种血清蛋白质/多肽(以下分别用A、B、C和D表示)区分结核病患者与健康人的能力。方法:应用表面增强激光解析-电离飞行时间质谱技术检测57例结核病患者和30例健康人的血清蛋白质谱,分析其A、B、C、D中的表达水平(用相对强度表示)并比较其差异,并应用诊断性试验评价方法分析其区分结核病患者群与健康人群的能力。结果:(1)A、B、C、D在结核病患者和健康人中的表达水平分别为1±11、1 597±3 102、460±765、1 208±1 003和123±201、47±98、36±93、397±355,两者差异显著。(2)以A、B、C、D血清表达水平为指标判定结核病患者和健康人的ROC曲线下面积分别为0.644、0.848、0.735和0.810,分界值分别为≤166、≥318、≥48和≥728。结论:B、C和D 3种血清蛋白质/多肽具有较好的区分结核病患者与健康人的能力,有望作为新型结核病诊断候选标志物。 Objective To assess the efficacy of using four kinds of proteins / peptides to distinguish the tuberculosis patients from healthy people. Methods A, B, C and D were used to represent four proteins /peptides with 1 060, 1 944, 2 081 and 3 954 of mass to charge ratio (m / z) in serum, respectively. Levels A, B, C and D in serum of 57 patients with tuberculosis and 30 healthy people were determined by using the surface-enhanced laser desorption-ionization time of flight mass spectrometry (SELDI-TOF-MS). Then the differences of levels of f A, B, C and D were anlyzed between tuberculosis patients and healthy people. The efficacy of distinguishing tuberculosis patient from healthy people were evaluated by using diagnostic test evaluation method. Results (1) The levels of A, B, C and D were 1 ± 11, 1 597 ± 3 102, 460 ± 765 and 1 208 ± 1 003 in tuberculosis patients, while they were 123 ± 201, 47 ± 98, 36 ± 93 and 397 ± 355 in healthy people. (2) The area under the receiver operator characteristic (ROC) curve was 0.644, 0.848, 0.735 and 0.810 respectively. The serum levels of A, B, C and D could be used to distinguish tuberculosis patient from healthy people and the cut-off values of A, B, C and D were ≤166, ≥318, ≥48 and ≥728, respectively. Conclusions B, C and D have better performances to distinguish tuberculosis patients from healthy people , which may be regarded as new promising candidate markers for diagnosis of tuberculosis.
出处 《实用医学杂志》 CAS 北大核心 2014年第23期3769-3771,共3页 The Journal of Practical Medicine
基金 国家“十二五”传染病防治重大科技专项(编号:2012ZX10004-903) 广州市科技计划项目(编号:2014Y2-00117)
关键词 结核病 蛋白质/多肽 诊断性试验评价 表面增强激光解析-电离飞行时间质谱 (SELDI-TOF-MS) Tuberculosis Protein/peptide Evaluation of diagnostic test Surface-enhanced laserdesorption-ionization time of flight mass spectrometry (SELDI-TOF-MS)
  • 相关文献

参考文献8

  • 1WHO. Global tuberculosis report 2013[J]. http: II www.who. int Itb Ipublications I global_report I en I.
  • 2Norbis L, Miotto P, Alagna R, et al. Tuberculosis: lights and shadows in the current diagnostic landscape[J]. New Microbiol, 2013,36(2):111-120.
  • 3GolubJE, Dowdy OW. Screening for active tuberculosis: methodological challenges in implementation and evaluation[J] . IntJ Tuberc Lung Dis, 2013,17(7) :856-865.
  • 4World Health Orgnization. Meetings: Tuberculosis. WHO, Geneva, Switzerland, 2008. www.who.int/tdr/news/2008/meetingstuberculosisl en/index l.html.
  • 5刘志辉,谭守勇,刘玉美,罗强生,云径平,李昕洁,刘健雄.结核病患者与健康人血清蛋白质谱的比较研究[J].结核病与肺部健康杂志,2012,1(3). 被引量:3
  • 6杨有业,张秀明.临床检验方法学评价[M].北京:人民卫生出版社,2008:118-127.
  • 7Fu YR, Yi ZJ, Guan SZ, et al. Proteomic analysis of sputum in patients with active pulmonary tuberculosis[J]. Clin Microbiol Infect, 2012,18 (12) : 1241-1247.
  • 8Song SH, Han M, Choi YS, et al. Proteomic profiling of serum from patients with tuberculosis[J]. Ann Lab Med, 2014, 34(5): 345-353.

二级参考文献10

  • 1Abebe F,Holm-Hansen C,Wiker HG. Progress in serodiagnosis of Mycobacterium tuberculosis infection[J].Scandinavian Journal of Immunology,2007,(2/3):176-191.
  • 2Cho SN. Current issues on molecular and immunological diagnosis of tuberculosis[J].Yonsei Medical Journal,2007,(03):347-359.
  • 3Steingart KR,Henry M,Laal S. Commercial serological antibody detection tests for the diagnosis of pulmonary tuberculosis:a systematic review[J].PLOS MEDICINE,2007,(06):e202.
  • 4Steingart KR,Henry M,Laal S. A systematic review of commercial serological antibody detection tests for the diagnosis of extrapulmonary tuberculosis[J].Postgraduate Medical Journal,2007,(985):705-712.
  • 5Steingart KR,Dendukuri N,Henry M. Performance of purified antigens for serodiagnosis of pulmonary tuberculosis:a meta-analysis[J].Clinical and Vaccine Immunology,2009,(02):260-276.
  • 6Sinchaikul S,Hongsachart P,Sriyam S. Current proteomic analysis and post-translational modifications of biomarkers in human lung cancer materials[J].Chang Gung Medical Journal,2008,(05):417-430.
  • 7Kon OL,Yip TT,Ho MF. The distinctive gastric fluid proteome in gastric cancer reveals a multi-biomarker diagnostic profile[J].BMC Med Genomics,2008.54-67.
  • 8Agranoff D,Fernandez-Reyes D,Papadopoulos MC. Identification of diagnostic markers for tuberculosis by proteomic fingerprinting of serum[J].The Lancet,2006,(9540):1012-1021.
  • 9温红侠,陈一强,何敏,王琪,冯晓凯,孔晋亮.采用蛋白芯片筛选活动性肺结核血清标志物[J].中华结核和呼吸杂志,2008,31(1):63-64. 被引量:4
  • 10吴雪琼,张俊仙,梁艳,董梅,易宾,马瑞娟,卫华,梁建琴,阳幼荣,陈红兵,张翠英,何菊芳,武红,李仲兴,刘又宁.应用蛋白质谱建立活动性肺结核病的血清诊断模型[J].中华微生物学和免疫学杂志,2008,28(11):1040-1043. 被引量:17

共引文献174

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部