漆姑草水提物对K562细胞凋亡及细胞周期影响

Regulating effects of Herba Saginae Japonicae on apoptosis and cell cycle of K562 cell strain in vitro

目的探讨漆姑草水提物(HSJ)对人白血病细胞株K562细胞凋亡及细胞周期影响。方法采用四甲基偶氮唑蓝(MTT)比色法检测HSJ对K562细胞的增殖抑制作用;台盼蓝拒染法观察HSJ对细胞生长曲线影响;流式细胞仪检测HSJ对K562细胞凋亡影响;DNA倍体分析法检测HSJ对K562细胞周期时相影响。结果 HSJ对K562细胞增殖抑制率随剂量增加而增加,作用48 h半数抑制浓度(IC50)为1.35 mg/m L;1.0、2.0、4.0 mg/m L HSJ处理后,K562细胞凋亡率分别为(8.39±0.81)%、(8.88±1.12)%、(13.42±2.04)%,均高于对照组的(4.60±1.09)%(P<0.05);HSJ能够引起细胞周期进程的改变,与对照组比较,HSJ增加K562细胞中G2期细胞比例(P<0.05),降低S期时相细胞比例(P<0.05),降低增殖指数(PI)(P<0.05),HSJ将K562细胞阻滞于G2期。结论 HSJ对K562细胞具有增殖抑制作用,其机制与诱导细胞凋亡和阻滞K562细胞在G2期有关。

Objective To investigate regulating effects of Herba Saginae Japonicae （HSJ） on apoptosis and cell cycle of K562 cell strain in vitro. Methods The inhibited proliferation of K562 cells was measured with methyl thiazolyl tetrazolium（MTT）. The effect of HSJ on cell growth was analzyed with trypan blue dye exclusion method. The HSJ-induced apoptosis of K562 cells was detected with annexinV-FITC/PI double staining method. The cell cycle phase of K562 cells was determined with DNA ploid analysis. Results There was a positive relation between inhibitive effect of HSJ on K562 cell proliferation and the concentration of HSJ. The 50% inhibitory concentration（ IC50 ） of HSJ was 1.35 mg/ml with the treatment of HSJ for 48 hours. The apoptotic ratios of K562 cells were 8.39 ±0. 81% ,8.88± 1.12%, and 13.42 ± 2. 04% treatd with HSJ of 1.0,2. 0, and 4. 0 mg/ml, respectively, with a significant difference compared with that of the control group（4. 60 ± 1.09 % ） （ P 〈 0. 05 ）. Compared with control, HSJ increased the percentage of the cells in G2 phase（P 〈 0. 05 ）, decreased the percentage of cells in S phase and the proliferous index（PI） （P 〈 0. 05, P 〈0. 05）. Cell cycle of K562 cells with HSJ treatment were blocked in G2 phase. Conclusion HSJ can inhibit the proliferation of K562 cells and the mechanism maybe involve the induced cell apoptosis and blocked G2 phase of K562 cells.