黄芩素对人肝癌HepG2细胞生长的抑制作用

Study on the Inhibitory Effect of Baicalein on the Growth of HepG2 Cell

目的:研究黄芩素对人肝癌Hep G2细胞生长的抑制作用。方法:在培养Hep G2细胞的96孔板上加入药物,使黄芩素的终浓度分别为0、20、40、80、160μmol/L(即对照组与黄芩素1、2、3、4组)。采用MTT法检测细胞的存活情况,分光光度法检测含半胱氨酸的天冬氨酸蛋白水解酶(Caspase)-3、8、9的活性,并检测丙二醛(MDA)含量,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果:与对照组比较,培养24、48、72 h时,黄芩素2、3、4组各时间点的吸光度降低;培养24 h时,Caspase-3、8、9活性增强,MDA含量增加,SOD、GSH-Px活性减弱,差异均有统计学意义(P<0.01)。结论:黄芩素对Hep G2细胞生长具有抑制作用,其机制与增强Caspase-3、8、9活性,增加MDA含量,减弱SOD、GSH-Px活性有关。

OBJECTIVE： To study the inhibitory effect of baicalein on the growth of HepG2 cell. METHODS ： The drugs were added into 96-well plate that cultivated HepG2 cells, so that the baiealein extract concentration were 0, 20, 40, 80, 160 μmol/L （namely blank control group and baicalein ①,②,③,④ group）. MTT method was adopted to detect cell inhibition ratio. Spectro- photometric method was adopted to detect the activity of Caspase-3, 8, 9; the content of MDA, the activity of SOD and GSH-Px were all determined. RESULTS： Compared with normal control group, the absorbance of baicalein group ②, ③, ④ decreased, the activity of 24 h Caspase-3, 8, 9, and the content of MDA increased; while the activity of SOD and GSH-Px decreased; there was statistical significance（P〈0.01 ）. CONCLUSIONS： Baicalin can inhibit the growth of HepG2 cells. The mechanism is related to the increase of inhibition ratio, the activity of Caspase-3,8,9 and the content of MDA, and the decrease of the activity of SOD and GSH-Px.