HPLC法测定不同产地凤仙花饮片中山奈素的含量

Content Determination of Kaempferol in Impatiens balsamina Decoction Pieces from Different Producing Areas

目的：建立测定不同产地凤仙花饮片中山奈素含量的方法。方法：采用高效液相色谱法。色谱柱为Diamonsil C18（250mm×4.6 mm,5μm）,流动相为乙腈-0.2%磷酸溶液（35∶65,V/V）,检测波长为360 nm,流速为1.0 ml/min,柱温为40℃,进样量为5μl。结果：山奈素的质量浓度在8.16～81.60μg/ml范围内与峰面积积分值呈良好线性关系（r=0.999 6）;精密度、重复性、稳定性试验的RSD〈2%;平均加样回收率为99.68%,RSD=1.9%（n=9）。结论：湖北宜昌所产的凤仙花饮片中的山奈素含量最高。该方法操作简便,结果准确、可靠,可用于凤仙花饮片的质量控制。

OBJECTIVE： To determine the content of kaempferol in Impatiens balsamina decoction pieces from different pro- ducing areas. METHODS： HPLC method was adopted. The determination was performed on Diamonsil C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of acetonitrile-0.2% phosphoric acid （35 ： 65, V/V） at the flow rate of 1.0 ml/min. The detec- tion wavelength was set at 360 nm, column temperature was 40 ℃, and sample size was 5 μl. RESULTS： The linear range of kaempferol were 8.16-81.60 μg/ml （r=0.999 6） with an average recovery of 99.68% （RSD=1.9%, n=9）. RSDs of precision, re- producibility and stability tests were all lower than 2 %. CONCLUSIONS： The content of kaempferol in I. balsamina decoction piec- es from Hubei Yichang is the highest. The method is accurate and reliable, and can be used for the quality control of I. balsamina decoction pieces. The method is accurate and reliable, and can be used for the quality control of I. balsamina decoction pieces.