摘要
采用单因素试验、部分因子析因试验、最陡爬坡试验及响应面试验对裂殖壶菌发酵培养基进行优化,优化后最佳培养基组成为葡萄糖52.5 g/L、酵母膏6.5 g/L、蛋白胨2 g/L、玉米浆7.5g/L、硫酸镁0.4g/L、磷酸二氢钾0.45 g/L、海水晶20 g/L。优化后裂殖壶菌生物量从优化前的4.33 g/L提高至15.88 g/L。
The objective of this study was to optimize the fermentation medium for Schizochytrium sp.ATCC 20888 by using single-factor experiment,fractional factorial design,path of steepest ascent and Box-Benhnken design.Based on the statistical analysis,the optimal medium was composed of glucose 52.5 g/L,yeast extract 6.5 g/L,peptone 2 g/L,corn steep powder 7.5 g/L,magnesium sulfate 0.4 g/L,potassium dihydrogcn phosphate 0.45 g/L,sea crystal 20 g/L.After optimization,the biomass increased from 4.33 g/L to 15.88 g/L.
出处
《中国饲料》
北大核心
2014年第23期22-25,共4页
China Feed
关键词
裂殖壶菌
培养基优化
响应面
Schizochytrium sp.ATCC 20888
medium optimization
response surface methodology
