枳术丸对功能性消化不良大鼠胃排空功能及Ghrelin、5-HT、CGRP的影响

Effects of Zhizhu pill on the gastric emptying function as well as Ghrelin,5-HT and CGRP in rats with functional dyspepsia

目的研究枳术丸对碘乙酰胺(iodoacetamide,IA)诱导的功能性消化不良(FD)大鼠胃排空功能及胃促生长素(Ghrelin)、5-羟色胺(5-HT)、降钙素基因相关肽(CGRP)的影响,初步探讨枳术丸治疗FD的机制。方法雄性10日龄SD幼鼠80只,随机分为正常组30只、造模组50只。采用0.1%IA灌胃诱导大鼠FD模型。大鼠8周龄时进行模型评定,将造模成功的50只大鼠随机分为模型组30只、枳术丸组10只和阳性药组10只。正常组与模型组予0.9%氯化钠溶液1 m L/100 g灌胃,枳术丸组予生药含量1.575 g/m L的水煎剂1 m L/100 g灌胃,阳性药组给予0.3 mg/m L多潘立酮溶液1 m L/100 g灌胃。采用苏木素-伊红(Hematoxin eosin,HE)染色观察大鼠胃组织病理变化;采用放射免疫法检测大鼠胃组织髓过氧化物酶(MPO)活性以及血清中Ghrelin、5-HT、CGRP的含量。结果枳术丸组和阳性药组大鼠3 h摄食量和胃排空率显著大于模型组(P<0.05),而3 h后胃内食物残余量小于模型组(P<0.05)。枳术丸组和阳性药组均可不程度地升高FD大鼠血清中Ghrelin和5-HT的含量(P<0.05,P<0.01),但2组间差异无统计学意义;枳术丸组和阳性药组均可不程度地降低FD大鼠血清中CGRP的含量(P<0.05),但2组间差异无统计学意义。结论枳术丸对IA诱导的FD大鼠具有加快胃排空、降低胃肠感觉过敏的作用,其机制可能与外周血Ghrelin、5-HT含量提高,CGRP含量降低有关。

Objective To study the effects of Zhizhu pill on the gastric emptying function as well as Ghrelin,5-HT and CGRP in rats with iodoacetamide-induced functional dyspepsia（ FD）,so as to explore the action mechanism of Zhizhu pill on FD. Methods 80 tenday-old male SD rats were randomly divided into a normal group（ n = 30） and a model establishment group（ n = 50）. The FD model was established by intragastric administration of 0. 1% iodoacetamide. Model evaluation of rats was performed at the age of 8 weeks,and 50 successful models of rat were randomly divided into a model group（ n = 30）,a Zhizhu pill group（ n = 10） and a domperidone group（ n = 10）. The normal group and model group were treated with intragastric administration of 0. 9% sodium chloride solution;Zhizhu pill group was treated with intragastric administration of Zhizhu pill; the domperidone group was treated with intragastric administration of domperidone. The hematoxylin eosin（ HE） staining was used to observe the pathological changes of gastric tissue in rats. Radioimmunoassay was used to detect the activity of MPO in gastric tissue and content of Ghrelin,5-HT and CGRP. Results The 3-h food consumption and gastric emptying rate in the Zhizhu pill group and domperidone group was significantly higher than those in the model group（ P〈0. 05）,and 3-h food residue was less than that in the model group（ P〈0. 05）. The Zhizhu pill group and domperidone group could increase the level of Ghrelin and 5-HT（ P〈0. 05）,but the differences between two groups were not significant. The Zhizhu pill group and domperidone group could reduce the level of CGRP（ P〈0. 05）,but the differences between two groups were not significant. Conclusion Zhizhu pill can promote the gastric emptying and reduce gastrointestinal hypersensitivity in FD rats induced by IA,and its mechanism might be related with the increasing of the Ghrelin and 5-HT and the reducing of CGRP.