消骨痛汤对骨关节炎大鼠TGFβ_1和BMP_2表达观察

The observation of Xiaogutong decoction in TGFβ_1 and BMP_2 of rats with osteoarthritis

目的观察中药方剂消骨痛汤对骨关节炎(OA)大鼠模型中软骨和滑膜中转化生长因子β1(TGFβ1)和骨形成蛋白2(BMP2)的表达。方法将40只Wistar大鼠随机分为4组,每组10只。正常对照组不予干预,其余3组采用膝关节腔内注射木瓜蛋白酶的方法建立骨关节炎大鼠模型,造模2周后开始给药,分别给予生理盐水(模型组)、双氯芬酸钠(西药组)和中药消骨痛汤(中药组),灌胃8周后取软骨及滑膜标本。用免疫组化方法检测TGFβ1和BMP2在软骨和滑膜中的蛋白表达。结果模型组和西药组软骨、滑膜中TGFβ1、BMP2水平低于中药组和正常组,差异均有统计学意义(P<0.05)。中药组和正常组TGFβ1和BMP2水平差异无统计学意义(P>0.05)。结论消骨痛汤能上调OA大鼠软骨和滑膜中TGFβ1和BMP2的蛋白表达。

Objective To observe the influence of Xiaogutong decoction in TGFβ1 and BMP2 of rats with osteoarthri-tis.Methods 40 cases rats were randomly divided into 4 groups,each of 10 cases.Normal control group with no intervention. The remaining 3 groups to establish osteoarthritis rat model.Dosing in 2 weeks after modeling.Respectively treated by normal saline,diclofenac and Xiaogutong decoction.After 8 weeks,taken the cartilage and synovial samples.TGFβ1 and BMP2 were de-tected by immunohistochemistry.Compared the level of TGFβ1 and BMP2 of 4 groups.Results The level of TGFβ1 and BMP2 in model group and western medicine group were lower than that of normal group and chinese medicine group,the difference was statistically significant（P〈0.05）.The level of TGFβ1 and BMP2 in normal group and chinese medicine group has no sta-tistically significant（P〉0.05）.Conclusion Xiaogutong decoction can improve the expression of the TGFβ1 and BMP2 pro-tein of cartilage and synovial of OA.