回肠间置术对减重和降糖作用的观察

Observe the effectiveness of ileal transposition for weight and blood glucose

目的 建立GK大鼠回肠间置术模型,探讨回肠间置术对GK大鼠进水量、进食量、体重及其血糖的影响.方法 将30只12周龄的雄性GK大鼠按随机数字表法随机分为3组：手术组（IT组）10只、假手术饮食控制组（C组）10只及假手术空白对照组（B）10只;IT组行回肠间置术,C组和B组行假手术,观察各组大鼠术前及术后饮水量、体重及血糖变化.结果 本实验总存活率86.7％;IT组大鼠进水量减少并保持在较低水平,IT组大鼠术后进食量和体重的变化一致,同B、C两组存在一过性差异;IT组较其他两组术后空腹血糖及OGTT均明显改善.结论 通过GK大鼠建立回肠间置术动物模型是可行的,发现回肠间置术对GK大鼠饮水量和血糖的控制是持久的,而对进食量和体重的影响是一过性的.

Objective Through developing the experimental animal model of ileal transposition （IT） with GK-rats,to explore the effects of ileal transposition on water and food intake,weight change and blood glucose.Methods Thirty male GK-rats were randomly assigned to three groups：IT（n =10）,C （n =10） and B （n =10）group.Rats in IT group underwent ileal transposition,and others underwent sham operation.Every group was given sufficient clean water.Water and food intake,weight change blood glucose and oral glucose tolerance test（OGTT） were recorded preoperatively and postoperatively.Results The total deaths of the experiment was four and the general survival rate was 88.6%.After operation,water intake reduced visibly in all groups,IT group was kept low level for a long time but not in C and B group.The food intake of IT group was lower than the other two groups during a certain period,but that phenomenon didnt last long.The weight change showed similarly to the food intake.The fasting plasma glucose（FPG） and OGTT were ameliorated significantly in IT group but not in B and C group.Conclusions It is feasible to develop ileal transposition animal models with GK-rats.Moreover,the author found that the influence of IT on weight in GK rats was transient,but on water intake and blood glucose was lasting.