基于Nafion-碳纳米管-球形二茂铁甲酸纳米复合物构建的电流型人绒毛膜促性腺激素免疫传感器的研究

Amperometric immunosensor based on nafion-multiwalled carbon nanotubes-spherical ferrocenecarboxylic acid nanocomposite for human chorionic gonadotrophin

该文采用超声溶剂置换方法合成球形二茂铁甲酸纳米粒子,以Nafion分散的多壁碳纳米管与球形二茂铁甲酸形成纳米复合物,并将该复合物固定在玻碳电极(GCE)表面,用EDC/NHS将二茂铁甲酸与L-半胱氨酸(L-Cys)交联,形成带有巯基官能团的复合膜,再键合沉积的纳米金(nano-Au),并通过纳米金吸附人绒毛膜促性腺激素抗体(anti-HCG),最后用牛血清蛋白(BSA)封闭电极上的非特异性吸附位点,从而制得人绒毛膜促性腺激素(HCG)免疫传感器。实验结果表明,该免疫传感器的响应电流与HCG的浓度有良好的线性关系,其线性范围为0.05 mIU/mL^200 mIU/mL,检出限为0.015 mIU/mL。

A novel electrochemical immunosensor was proposed based on Nafion-multiwalled carbon nanotubespherical ferrocenecarboxylic acid nanocomposite （Nafion-MWCNT-FcCOOH） for determination of human chorionic gonadotrophin （HCG）. Spherical ferrocenecarboxylic acid nanoparticles were prepared by the ultrasonic-solvent-substitution method. The nanocomposite was fabricated on the surface of the bare glassy carbon electrode and used as substrate for construction of amperometric immunosensor. Under optimal experimental conditions, the proposed immunosensor exhibited a good response to HCG, the linear working range spanned the concentrations of HCG from 0.05 mIU/mL to 200 mIU/mL with a detection limit of 0.015 mIU/mL.