非光合固碳微生物菌群的耐盐特性及其影响因素

Salt-tolerant characteristics of non-photosynthetic microbial community and its influencing factors

从海洋中分离驯化得到的非光合固碳微生物菌群(NPMC)是无需光照和供氢的化能自养微生物,若能用于贫瘠盐碱地改良,实现其在盐碱土壤中的二次固碳,对于盐碱土壤的低碳化改良具有重要的意义。本实验初步验证了NPMC的耐盐特性,以及微量元素和磷酸盐缓冲液两单因素在次高盐条件下对NPMC固碳效率的影响。并通过响应面法研究了微量元素与盐浓度对NPMC固碳效率的交互作用。结果表明,非光合固碳微生物拥有耐受高盐浓度的特性,可耐受高达100g/L以上的总盐度,因此可用于重盐碱土壤的改良。微量元素和磷酸盐缓冲液浓度的增加,都可增强NPMC的固碳效率,微量元素的促进效应高于磷酸盐缓冲液。微量元素和盐度对NPMC固碳效率的影响存在交互作用。

The non-photosynthetic microbial community （NPMC） isolated from ocean are chemoautotroph microbes with- out providing photosynthetic and hydrogen. Using NPMC to improve alkali-saline land can achieve its secondary carbon fixation in saline soil and thus reach low-carbon improvement to saline-alkali soil. In this experiment, the salt-tolerance feature of NPMC was evaluated, and the effects of trace elements and phosphate buffer on the CO2 fixation efficiency of NPMC under sub-high-salt condition were also studied, respectively. The results showed that NPMC could tolerate high salt concentration up to 100 g/L, which was enough for the heavy saline-alkali land reclamation. Trace elements and phos- phate buffer could enhance the CO2 fixation efficiency of NPMC, respectively. Under the sub-high-salt condition, trace elements was more effective than phosphate buffer. In addition, there was interaction between trace elements and salinity for the CO2 fixation efficiency of NPMC.