紧密连接蛋白－1咬合蛋白及白细胞介素－18在急性重症胰腺炎肠黏膜中的表达

ZO -1, Occludin, IL -18 expression in intestinal mucosa of patients with severe acute pancreatitis

目的：观察急性重症胰腺炎（ severe acute pancreatitis ， SAP）肠黏膜紧密连接蛋白－1（ZO－1）、肠上皮咬合蛋白（Occludin）及白细胞介素（IL）－18的表达变化，探讨急性重症胰腺炎时肠道屏障功能损伤的机制。方法选择2012－06-2012－12在我院消化科住院治疗的急性重症胰腺炎患者和急性轻型胰腺炎患者各30例为胰腺炎组。选取正常健康人30例作为对照组。在急性胰腺炎患者置鼻空肠营养管同时，采集距离十二指肠乳头5 cm以上对侧十二指肠黏膜组织。采用免疫组织化学SP法检测肠组织ZO－1、Occludin、IL－18蛋白表达，采用Western blotting法进行肠组织ZO－1、Occludin、IL－18蛋白半定量检测。结果对照组、轻型胰腺炎组、重症胰腺炎ZO－1阳性率分别为56．7％、50．0％、40．0％，轻、重胰腺炎组ZO－1阳性率低于对照组（P＜0．05）。重症胰腺炎组ZO－1阳性率低于轻型胰腺炎组（P＜0畅05）。对照组及轻、重症胰腺炎组Occludin阳性率分别为50．0％、40．0％、33．3％，轻、重症胰腺炎组Occludin阳性率低于对照组（P＜0．05），重症胰腺炎组Occludin阳性率低于轻型胰腺炎组（P＜0．05）。对照组、轻型胰腺炎组、重症胰腺炎组IL－18阳性率分别为10．0％、46．7％、86．7％，轻、重症胰腺炎组IL－18阳性率高于对照组（P＜0．05），重症胰腺炎组IL－18阳性率高于轻型胰腺炎组（P＜0畅05）。结论急性重症胰腺炎时，ZO－1、Occludin表达降低，IL－18表达增高，可能共同参与了急性胰腺炎肠黏膜屏障功能损伤的病理过程。

Objective To investigate the intestinal expression of tight junction protein ZO -1, Occludin and IL-18 , and the mechanisms of intestinal barrier dysfunction in severe acute pancreatitis . Methods The present study enrolled 30 cases of severe acute pancreatitis and 30 cases of mild acute pancreatitis who were hospitalized in our hospital from June 2012 to December 2012 .We also included 30 cases of normal healthy people as a control group .When set up nasal jejunal feeding tube for the pancreatitis patient , duodenal mucosa was sampled on the opposite side that was 5 cm far away from the duodenal papillary . The expression of ZO -1, Occludin and IL -18 were detected by immunohistochemistry and semi -quantitative detected by Western blotting .Results ZO-1 positive rates were 40.0%, 50.0%and 56.7% in the severe pancreatitis, mild pancreatitis and control group respectively, with significance difference among the groups （P〈0.05）.Occludin-positive rates were 33.3%, 40.0%and 50.0%in the severe pancreatitis , mild pancreatitis and control group respectively ,with significance difference among the groups （P〈0.05）.IL-18 positive rates were 86.7%, 46.7%and 10 .0%in the severe pancreatitis , mild pancreatitis and control group respectively , with significance difference among the groups （P〈0.05）.Pancreatitis IL-18 positive rate was higher than the control （P〈0.05）, and severe pancreatitis showed a higher IL -18 positive rate compared with the mild pancreatitis （ P 〈0.05）.Conclusion Decreased expression of ZO -1 and Occludin, increased expression of IL-18 , may be involved in the pathogenesis of acute intestinal barrier function injury .