胰岛素样因子3应用于男性迟发性性腺功能减退诊断的可行性研究

Study of INSL3 for the clinical application in the diagnosis of male late onset hypogonadism

目的分析胰岛素样因子3(insulin like factor 3,INSL 3)应用于男性迟发性性腺功能减退(late onset hypogonadism,LOH)诊断的可行性。方法选取中老年男性,间隔1个月抽取空腹晨血2次,分析INSL 3在血清中的平均浓度及其稳定性;依据男性LOH的诊断标准,分析患者组与正常对照组之间血清中INSL 3的浓度是否存在差异。结果接受重复抽血的中老年男性44人,血清INSL 3的浓度均值为(1.86±0.64)ng/m L,经过1个月的间隔期血清中INSL 3的浓度变化差异无统计学意义(P>0.05)。在患者组与正常对照组之间血清中INSL 3的浓度差异有统计学意义(P<0.05)。进一步研究发现血清INSL 3浓度与促黄体生成素(luteotropic hormone,LH)、总睾酮、游离睾酮之间存在相关性(P<0.05),而与绝育术、烟酒、糖尿病、高血压、冠心病、体质量指数和中老年男性症状量表(aging males’symptoms scale,AMS)评分之间无相关性(P>0.05)。结论初步研究提示血清INSL 3与男性性腺功能状态紧密相关且表达稳定,有望用于LOH的诊断。

Objective To analyze the suitability of insulin like factor 3（ INSL3） for the clinical application in the diagnosis of male late onset hypogonadism（ LOH）. Methods The ageing men were recruited and sampled the fasting blood twice with one month interval,and the INSL3 was tested for the blood samples to study its stability in blood. According the diagnosis criteria of LOH,the difference of INSL3 concentration between the LOH patient group and normal control group was analyzed. Results 44 aging males sampled the blood twice. The INSL3 concentration in serum is（ 1. 86 ± 0. 64） ng / m L and the fluctuation with one month interval with no statistical significance（ P 〉 0. 05）; The INSL3 concentration in serum between two groups with statistical significance（ P 〈 0. 05）;The further study found that there is an association between the concentration correlation of INSL3 with LH and total testosterone（ TT）and free testosterone（ c FT）（ P 〈 0. 05）; There is no association with male ligation,tobacco,alcohol,diabetes,hypertension,coronary heart disease,body mass index（ BMI） and aging males symptoms scale（ AMS） score（ P 〉 0. 05）. Conclusion The study suggests that INSL3 has a clear- cut correlation with sexual performance and maybe adopted in the diagnosis of LOH.