热毒宁对呼吸道合胞病毒感染哮喘模型小鼠的影响

Effect of Reduning on Respiratory Syncytial Viruses Induced Airway Inflammation and Asthma Exacerbation in Mice

目的:探讨热毒宁(RDN)对呼吸道合胞病毒(RSV)感染哮喘加重小鼠气道炎症的影响。方法:雌性BALB/c小鼠32只,随机分成4组,分别为对照组、鸡卵白蛋白(OVA)组、OVA/RSV组、OVA/RSV/RDN组。无创肺功能检测各组小鼠气道反应性;HE染色观察肺部炎症变化;小鼠支气管肺泡灌洗液(BALF)中炎性细胞计数并分类;ELISA法检测BALF中IL-4、IL-5、IL-13、IFN-γ含量。结果:RSV感染能够加重哮喘小鼠气道炎症与气道高反应性,RDN抑制RSV感染哮喘加重小鼠气道高反应性(P<0.05);RDN显著减少RSV感染哮喘小鼠BALF中IL-4、IL-5、IL-13、IFN-γ含量。结论:RDN可以减轻RSV感染的小鼠气道炎症反应,抑制气道高反应性。

Objective： To evaluate the effect of Reduning （RDN） on respiratory syncytial virus （RSV）-induced airway inflammation and asthma exacerbation in Balb/c mice. Methods： Thirty-two female mice were randomly divided into 4 groups （n=8 for each group）： control group, OVA （ovalbumin） group, OVA/RSV group and OVA/RSV/RDN group. Airway responsiveness was assessed using a whole body plethysmog_raphy. Lung histopathological changes were evaluated by HE stain. Cells in bronchoalveolar lavage fluid （BALF） were counted and classified, the supernatants of the BALF were used for detection of interleukin （IL） -4, IL-5, IL-13 and interferon （IFN）-γ. Results： RSV infection induced airway inflammation and air_way hyper-responsiveness （AHR） exacerbation. Administration of RDN remarkably inhibited airway inflam_mation and AHR. IFN-γ, IL-4, IL-5 and IL-13 levels in BALF decreased in OVA/RSV/RDN group com_pared with OVA/RSV group （P〈0.01, respectively）. Conclusion： RDN might suppress airway inflammation and AHR in RSV infected asthmatic mice.