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热毒宁对呼吸道合胞病毒感染哮喘模型小鼠的影响 被引量:7

Effect of Reduning on Respiratory Syncytial Viruses Induced Airway Inflammation and Asthma Exacerbation in Mice
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摘要 目的:探讨热毒宁(RDN)对呼吸道合胞病毒(RSV)感染哮喘加重小鼠气道炎症的影响。方法:雌性BALB/c小鼠32只,随机分成4组,分别为对照组、鸡卵白蛋白(OVA)组、OVA/RSV组、OVA/RSV/RDN组。无创肺功能检测各组小鼠气道反应性;HE染色观察肺部炎症变化;小鼠支气管肺泡灌洗液(BALF)中炎性细胞计数并分类;ELISA法检测BALF中IL-4、IL-5、IL-13、IFN-γ含量。结果:RSV感染能够加重哮喘小鼠气道炎症与气道高反应性,RDN抑制RSV感染哮喘加重小鼠气道高反应性(P<0.05);RDN显著减少RSV感染哮喘小鼠BALF中IL-4、IL-5、IL-13、IFN-γ含量。结论:RDN可以减轻RSV感染的小鼠气道炎症反应,抑制气道高反应性。 Objective: To evaluate the effect of Reduning (RDN) on respiratory syncytial virus (RSV)-induced airway inflammation and asthma exacerbation in Balb/c mice. Methods: Thirty-two female mice were randomly divided into 4 groups (n=8 for each group): control group, OVA (ovalbumin) group, OVA/RSV group and OVA/RSV/RDN group. Airway responsiveness was assessed using a whole body plethysmog_raphy. Lung histopathological changes were evaluated by HE stain. Cells in bronchoalveolar lavage fluid (BALF) were counted and classified, the supernatants of the BALF were used for detection of interleukin (IL) -4, IL-5, IL-13 and interferon (IFN)-γ. Results: RSV infection induced airway inflammation and air_way hyper-responsiveness (AHR) exacerbation. Administration of RDN remarkably inhibited airway inflam_mation and AHR. IFN-γ, IL-4, IL-5 and IL-13 levels in BALF decreased in OVA/RSV/RDN group com_pared with OVA/RSV group (P〈0.01, respectively). Conclusion: RDN might suppress airway inflammation and AHR in RSV infected asthmatic mice.
出处 《药学与临床研究》 2014年第6期499-501,共3页 Pharmaceutical and Clinical Research
基金 江苏省卫生厅医学科技发展基金临床药学科研课题(奥赛康基金课题)(P200960) 江苏省中医药管理局科研项目(LZ13228) 南京市卫生局科研项目(YKK13176)
关键词 呼吸道合胞病毒 哮喘 气道炎症 热毒宁 Respiratory syncytial viruses Asthma Airway inflammation Reduning
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