人参二醇组皂苷增强紫杉醇对人卵巢癌细胞株SKOV3细胞凋亡的研究

Study on the effect of paclitaxel on apoptosis of human ovarian cancer SKOV3 cells enhanced by Panaxadiol saponins

目的:探讨人参二醇组皂苷(PDS)与紫杉醇(PTX)联合应用对DUl45前列腺癌细胞增殖及凋亡的影响。方法:MTT法检测PTX单独组、PTX+地塞米松(Dex)组和PTX+PDS组SKOV3卵巢癌细胞存活的情况;流式细胞仪检测各组细胞凋亡情况;免疫印迹方法检测各组细胞活化形式caspase-3蛋白和AKT磷酸化的表达情况。结果:紫杉醇单独作用能显著的以浓度依赖性的方式降低细胞的存活,联合应用Dex后存活的SKOV3细胞数明显增加,PDS可以增加PTX对SKOV3细胞的增殖抑制作用。流式细胞仪检测细胞凋亡结果发现,PTX处理SKOV3细胞,凋亡细胞数约占总细胞数的22.38%,PTX联合Dex组其凋亡细胞数下降到总细胞数的14.52%(P<0.05),PTX联合PDS组其凋亡细胞数增加至39.54%。在SKOV3细胞中,PTX能显著诱导活化的caspase-3的表达,而Dex能够明显抑制PTX对活化的caspase-3表达的诱导作用。PDS可以促进活化的caspase-3的表达。与PTX组比较,PTX+Dex组AKT的磷酸化水平升高,而PDS可以使SKOV3细胞中的AKT的磷酸化显著降低。结论:PDS通过增强化疗药物PTX对SKOV3细胞的凋亡作用来增强对肿瘤细胞的杀伤作用。

Objective： To explore the effect of Panaxadiol saponins（ PDS） combined with paclitaxel on proliferation and apoptosis of ovarian cancer SKOV3 cells. Methods： MTT assay was used to detect the survival rates of human ovarian cancer SKOV3 cells in paclitaxel group,paclitaxel ＋ dexamethasone group and paclitaxel ＋ PDS group; flow cytometry was used to detect apoptosis of human ovarian cancer SKOV3 cells in the three groups; Western blot was used to detect caspase-3 protein expression and p-AKT expression in the three groups. Results： Paclitaxel could significantly reduce survival rate of human ovarian cancer SKOV3 cells in a dose-dependent manner. The survival rate of human ovarian cancer SKOV3 cells in paclitaxel ＋ dexamethasone group increased significantly,PDS could enhance the inhibiting effect of paclitaxel on proliferation of paclitaxel. The apoptotic rates of human ovarian cancer SKOV3 cells in paclitaxel group and paclitaxel ＋ dexamethasone group were 22. 38% and 14. 52%,respectively,there was statistically significant difference between the two groups（ P 0. 05）; in paclitaxel ＋ PDS group,the apoptotic rate increased to 39. 54%. Paclitaxel could significantly induce expression of activated caspase-3 in human ovarian cancer SKOV3 cells,but dexamethasone could significantly inhibit the induction effect of paclitaxel on expression of activated caspase-3 in human ovarian cancer SKOV3 cells. PDS could promote the expression of activated caspase-3. Compared with paclitaxel group,p-AKT expression level in paclitaxel ＋ dexamethasone group increased,but PDS could significantly reduce p-AKT expression level in human ovarian cancer SKOV3 cells. Conclusion： PDS can enhance cell-killing effect by strengthening apoptotic effect of paclitaxel on ovarian cancer SKOV3 cells.