Survivin在卵巢癌细胞顺铂耐药中的作用

The role of Survivin in drug resistance of ovarian cancer cells to cisplatin

目的:探讨Survivin在卵巢癌顺铂耐药中的作用。方法:应用RT-PCR和Western Blot检测卵巢癌顺铂敏感细胞株SKOV3和耐药株SKOV3/DDP中survivin表达,MTT法检测细胞增殖抑制率,流式细胞技术检测卵巢癌细胞的凋亡率,Survivin反义寡核苷酸被脂质体转染至SKOV3/DDP细胞中。结果:SKOV3/DDP中Survivin mRNA的相对表达强度为(1.21±0.59),显著高于SKOV3细胞中(0.54±0.23),差异有统计学意义(P<0.05)。Survivin蛋白在两种细胞的相对表达强度分别是SKOV3细胞(0.87±0.48)和SKOV3/DDP细胞(2.07±1.01),差异有统计学意义(P<0.05)。Survivin-ASOD可降调卵巢癌细胞中Survivin的表达,并显著增加细胞对顺铂敏感性。结论:卵巢癌细胞对顺铂耐药可能与SKOV3/DDP细胞中Survivin表达增加有关。

Objective： To explore the role of Survivin in drug resistance of ovarian cancer cells to cisplatin. Methods： RT-PCR and Western Blot were used to detect the expressions of Survivin in ovarian cancer SKOV3 cells sensitive to cisplatin and ovarian cancer SKOV3 / DDP cells resistant to cisplatin. MTT was used to detect the inhibition rate of cell proliferation,flow cytometry was used to detect the apoptotic rate of ovarian cancer cells; Survivin antisense oligodeoxynucleotide was transfected into SKOV3 / DDP cells with liposome. Results：The relative expression intensity of Survivin mRNA in ovarian cancer SKOV3 / DDP cells was（ 1. 21 ± 0. 59）,which was statistically significantly higher than that in ovarian cancer SKOV3 cells（ 0. 54 ± 0. 23）（ P 0. 05）. The expression intensities of Survivin protein in ovarian cancer SKOV3 cells and ovarian cancer SKOV3 / DDP cells were（ 0. 87 ± 0. 48） and（ 2. 07 ± 1. 01）,respectively,there was statistically significant difference（ P 0. 05）. Survivin-ASOD could down-regulated the expression of Survivin in ovarian cancer cells and significantly strengthened the sensitivity of ovarian cancer cells to cisplatin. Conclusion： Drug resistance of ovarian cancer cells to cisplatin may be correlated with up-regulation of Survivin in ovarian cancer SKOV3 / DDP cells.