1950 MHz GSM-Talk信号对睾丸支持细胞增殖和分泌功能的影响

Effects of 1950 MHz GSM-Talk signal on sertoli cells proliferation and secretion

采用贴壁生长的小鼠睾丸支持细胞(TM4)在含10%胎牛血清的DMEM/F12 1:1培养液中培养至指数生长期后,消化制备成1.5×103个·m L-1的细胞悬液,接种于35 mm培养皿中,每皿接种3 m L,接种12个皿。细胞接种后24 h更换新鲜培养液,然后将培养皿随机分为假辐照组和辐照组,每组6个皿。将2组细胞分别置于辐照装置的2个小室中,其中一个小室产生GSM-Talk信号,1950 MHz连续波,比吸收率为3 W·kg-1,辐照时间为5 d;另一个小室不产生GSM信号,用于假辐照。辐照结束后1-5 d,采用CCK-8和Brd U法检测细胞的增殖情况,免疫荧光染色检测细胞增殖标记物Ki67的蛋白表达,ELISA法检测小鼠干细胞因子(SCF)和小鼠胶质细胞系来源的神经生长因子(GDNF)的水平。结果显示:与对照组相比,辐照组TM4细胞的增殖能力明显减弱(p<0.05),细胞增殖标记物Ki67的表达亦有减弱趋势,细胞上清中SCF水平明显降低(p<0.05),而GDNF水平明显升高(p<0.05)。实验结果提示,GSM-Talk连续辐照5 d可抑制TM4细胞的增殖并影响其分泌功能。

The aim is to investigate the effects of 1950 MHz radiofrequency radiation （GSM-Talk Signal） on mouse sertoli cells proliferation and secretion. The adherent TM4 cells were cultured in DMEM/F 12 1：1 containing 10% FBS. Exponentially growing cells were digested to cell suspension of 1.5 × 103 cells·mL^-1 and then cultured in 35 mm petri dishes with 3 mL for each dish, 12 dishes in total. After incubation for 24 h, medium was changed and the dishes were randomly divided into sham-exposure group and exposure group （GSM-Talk signal, 1950 MHz continuous wave, the specific absorption rate was 3 W＇kg-1） with 6 dishes for each group. The dishes were placed in exposure waveguide chamber and sham-exposure waveguide chamber. After exposure for 5 d, the proliferation of cells was evaluated by CCK-8 and BrdU methods. The expression of cell proliferation marker Ki67 was measured through immunofluorescence staining. The levels of mouse stem cell factor （SCF） and mouse glial cell line-derived neurotrophic factor （GDNF） in cell supernatants were detected by ELISA assay. The results showed that compared with sham-exposure group, the proliferation of TM4 cells in exposure group significantly decreased（p〈0.05）, and the Ki67 level also tended to be reduced; the level of SCF in cell supernatants was lower （p〈0.05） while the level of GDNF was higher than that of sham-exposure group （p〈0.05）. The results suggest that the GSM-Talk signal exposure for 5 d could affect the proliferation and secretion of TM4 cells.