高产葡萄糖耐量因子(GTF)酵母菌株的筛选及中试工艺

Screening of High-yield Glucose Tolerance Factor Yeast and Study on Pilot Plant Scale Test

为研究高产葡萄耐量因子醇母菌在生产过程中富铬性能的变化,本文通过铬耐量试验和富铬能力筛选试验,从9株供试啤酒酵母菌中获得1株富铬能力强,遗传性能稳定,综合指标高的耐铬性菌株。利用摇瓶发酵工艺,通过15L和50L逐级放大中试生产工艺对筛选菌株富铬能力进行了研究。结果表明,经过50L发酵罐发酵工艺,菌株（YSI-3.7）有机铬含量为1 234μg·g-1,总铬含量为1 502μg·g-1,有机铬百分含量为82%,干菌体生物量为0.96g·100mL-1发酵液,湿菌体生物量为6.00g·100mL-1发酵液。菌株在中试生产过程中保持较高的富铬性能,其富铬能力是市售富铬酵母产品的2~5倍。该研究表明菌株YSI-3.7可作为GTF产业化生产供试菌株,为GTF产业化生产提供研究基础。

In order to study the ability to enrich chromium in pilot plant scale test by high-yield yeast stain, In this study, one of the better enrich ability of chromium, stable performance, and high biomass strains was screened from 9 tested brewer＇s yeast strains through chromium tolerance test and the ability to enrich chromium screening experiment. Based on shake flask fermentation experiment, We have studied the ability enrich chromium of the strain through the pilot plant scale test from 15 L to 50 L. The results show that, after 50L fermentation the strain content of organic chromium was 1234μg.g^-1 and that of total chromium was 1502μg.g^-1, and the ratio of organic chromium was 82% ,the biomass was 0.96g.100mL-1dry cells and 6.00g. 100mL^-1 wets cell. The strain maintain high ability enrich chromium in pilot experiments. It＇s 2- 5 times to product organic chromium compared with the commercially available chromium rich yeasts. This study showed that YSI - 3.7 can be used as strains of GTF production and has provided basis studies of GTF industrial production.