期刊文献+

蓝莓多酚对巨噬细胞IL-1β、IL-6基因表达的影响 被引量:2

Effects of the Blueberry Polyphenols on IL-1β,IL-6 Gene Expression in Mice Macrophages
下载PDF
导出
摘要 本试验通过测定EPP和NEPP对IL-1β和IL-6mRNA表达的影响,确定蓝莓可萃取多酚(EPP)及不可萃取多酚(NEPP)是否具有抗炎作用.结果表明,蓝莓中EPP和NEPP均能抑制细胞中IL-1β、IL-6 mRNA的表达,EPP的抑制效果要优于NEPP.当培养时间为48 h时,EPP和NEPP的添加浓度为10~ 200μg·mL-1对IL-1β和IL-6 mRNA抑制作用明显.当EPP的添加浓度为100μg·mL-1时,在12 ~48 h培养时间内,对IL-1β和IL-6 mRNA抑制作用明显;而100μg· mL-1 NEPP处理组中,在48h时对IL-1β和IL-6 mRNA抑制效果明显.这表明从蓝莓中的NEPP同EPP一样可通过抑制IL-1β、IL-6 mRNA的表达表现出明显的抗炎作用,对今后蓝莓在医药保健方面研究奠定基础,不可萃取多酚的研究为多酚研究提供了新的方向. The effects of EPP and NEPP from blueberries on mRNA expression levels of IL - 1β and IL -6 in LPS- induced RAW 264.7 cells were studied in the present paper. The results showed that the mRNA expression levels of IL - 1β and IL - 6 in macrophages were decreased by EPP and NEPP, and the inhibition effect of EPP was better than that of NEPP. The strong inhibition effect on IL- 1β and IL-6 mRNA expression was detected in 10 -200 μg·mL-1 EPP or NEPP, at 48 h. In the treatment of 100 μg· mL -1 EPP, the significant inhibition effect on IL- 113 and IL- 6mRNA expression was detected at 12-48 h, and the significant inhibition effect on IL-6mRNA expression was detected at 48 h in the treatment of 100 μg·mL -1 NEPP. It is suggested that EPP and NEPP from blueberries have significant anti- inflammatory effect by inhibiting expression levels of IL - 1β and IL - 6 mRNA in macrophages.
出处 《核农学报》 CAS CSCD 北大核心 2014年第10期1828-1833,共6页 Journal of Nuclear Agricultural Sciences
基金 国家自然科学基金青年项目(31101270)
关键词 蓝莓 可萃取多酚 不可萃取多酚 巨噬细胞 细胞因子 Blueberry Extractable Polyphenols Non-extractable Polyphenols Macrophages Cytokines
  • 相关文献

参考文献20

  • 1Peter C H, Ilja C W. Flavonols, flavones and flavanols-nature, occurrence and dietary burden [ J ]. Science of Food and Agriculture, 2000, 80(7 ) : 1081 - 1093.
  • 2吕业春,刘翼翔,吴薇,周峰,籍保平,苏春元.蓝莓多酚对油酸诱导HepG2细胞脂肪累积的干预作用[J].食品科学,2011,32(17):308-312. 被引量:17
  • 3陈杭君,李兴飞,郜海燕,房祥军.山核桃仁多酚组分分析及抗氧化研究[J].核农学报,2013,27(1):61-67. 被引量:18
  • 4Lau F C, Joseph J A, McDonald J E, Kalt W. Attenuation of iNOS and COX2 by blueberry polyphenols is mediated through the suppression of NF -κB activation. Journal of Functional Foods [ J] , 2009,1 ( 3 ) :274- - 283.
  • 5Seeram N P. Berry fruits for cancer prevention: Current status and future prospects [ J]. Journal of Agricultural and Food Chemistry, 2008, 56(3) :630 -635.
  • 6Giovanelli G, Buratti S. Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties [ J ]. Food Chemistry, 2009, 112 (4) :903 - 908.
  • 7Kristl J, Slekovec M, Tojnko S, Unuk T. Extractable antioxidants and non-extractable phenolics in the total antioxidant activity of selected plum cultivars ( Prunus domestiea L. ) : Evolution during on-tree ripening [J]. Food Chemistry, 2011, 125(1):29-34.
  • 8Dinaralla D A. Interleukin - 1 and its biologically related cytokine [ J]. Advances in Immunology, 1989, 44 : 153 - 205.
  • 9Ohta K, Pang X P, Berg L, Hershmao J M. Antitumor action of cytokines on new human papillary thyroid carcinoma cell lines [ J].Clinical Endocrinology & Metabolism, 1996, 81 (7) :2607 - 2612.
  • 10Haznedaroglu I C, Ozcebe O I, Ozdemir O, Celik I, Dundar S V, Kirazli S. Impaired haemostatic kinetics and endothelial function in Behcets disease [ J ]. Internal Medicine, 1995, 55 (5) : 339 - 340.

二级参考文献88

共引文献77

同被引文献39

引证文献2

二级引证文献21

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部