基于454测序的碧桃花瓣组织转录组核苷酸变异

Identification and Characterization of Nucleotide Variations in the Transcriptome of Ornamental Peach Flower Petals Based on 454 Sequencing

通过对碧桃花瓣组织进行转录组测序,获得1 556 684条序列,平均读长446 bp,共计695.34 Mb数据,其中1 492 289条高质量序列经拼接后得到22 762个重叠群。以拼接后的重叠群作为参考序列,利用SNP/In Del分析软件检测碧桃花瓣组织转录组中的核苷酸变异位点,共得到9 836个SNP和1 550个In Del位点。在SNP位点中,转换占62.55%、颠换占37.45%,A/G的变异最为丰富,C/G的变异最少。在In Del位点中,有34.71%的位点为单核苷酸插入、缺失突变。

We identified single-nucleotide polymorphisms （SNP） and insertion deletion length polymorphism （InDel）in the transcriptome from ornamental peach flower petals using the sequences generated by the Roche 454 GS-FLX sequencer .A total of 1 556 684reads were generated with an average length of 446 bp.After filtering poor quality reads , 1 492 289 high quality reads were obtained and were further assembled into 22 762 contigs .Using these contigs as a reference sequences , 9 836 SNPs and 1 550 InDels were identified.In the SNPs,transitions and transversions were accounted for 62.55%and 37 .45%, respectively.In these SNPs, A/T transitions were the most abundant , and C/G transitions were much rarer than any of the other SNP types. In the InDels, mononucleotide changes amounted for 34.71%.