副溶血性弧菌分子标志基因研究概况

Molecular genetic makers for Vibrio parahaemolyticus-A review

副溶血性弧菌是重要的食源性致病菌,其中,O3:K6血清型是1996年后导致多个国家多起食物中毒暴发的病原菌。中国1992-2001年的统计数据表明,由副溶血性弧菌引起的胃肠炎占由微生物引起的食源性疾病暴发的31.1%。副溶血性弧菌环境株大部分是非致病性的,而临床株则能产生耐热直接溶血素、耐热相关溶血素以及其它毒力因子。本文综述了3种重要的副溶血性弧菌的分子标志物,包括种特异性基因、毒力基因以及大流行菌群特异基因,旨在为研究者们针对性的选取基因开展快速检测副溶血性弧菌和鉴别其致病因子的研究提供参考依据。

Vibrioparahaemolyticus is an important foodborne pathogen, of which the 03 ： K6 serotype caused many outbreaks in different countries since 1996. Based on the 10 years data （1992-2001） from China, gastroenteritis caused by Vibrio parahaemolyticus accounted for 31.1% of foodborne disease outbreaks that were resulted from microorganisms. Most environmental strains of Vibrioparahaemolyticus are non-pathogenic strains. However, clinical strains can producethermostable direct hemolysin （TDH）, TDH-related hemolysin, and other virulence factors. Here we reviewed three commonly used molecular markers forVibrioparahaemolyticus, including species-specific genes, the virulence genesand pandemic group-specific genes, so that to provide references for the rapid detection of Vibrio parahaemolyticus and the identification of its pathogenic factor.