牛瘤胃乳酸菌的分离与鉴定

Isolation and Selection of Lactic Acid from Bovine Rumen

为分离筛选用于牛活菌制剂的乳酸菌菌株,本试验采用API 50CHL对从牛瘤胃中分离的60株乳酸菌进行生化鉴定,对鉴定出的菌株进行耐酸、耐碱、耐氧、生长温度、耐药和抑菌性测定。结果显示,经生化鉴定出的3株乳酸菌分别为嗜酸乳杆菌（L.acidophilus）、发酵乳杆菌（L.fermentum）和乳明串珠菌（L.holzapfelii）,分别命名为L1、L2和L3。其中,L1耐酸性最强,L2耐碱性最强,L1在37-40℃均生长良好且兼性厌氧;L1、L2、L3耐药率分别为80%、67%、53%;3株乳酸菌对大肠杆菌和金黄色葡萄球菌均有不同程度的抑制作用;L1抑菌效果最好,抑菌直径为18.3mm和16.4mm。结果表明,L1、L2可作为抗性乳酸菌活菌制剂的候选菌株。

To select and separate viable preparations for bovine strains of lactic acid bacteria,this separated lactic acid bacteria from bovine rumen were identified by API 50 CHL, the 3 identified strains were detected by alkali resistance, oxygen, growth temperature, drug resistance and bacteriostatic experiments they were L.acidophilus（L1）, L.fermentum（L2） and L.holzapfelii（L3）. The results showed that L1 had strongest acid resistance, L2 had strongest alkali resistance, L1 was grown strongly in 37℃-40℃ and facultative anaerobic. The resistivity rate of L1,L2,L3 was 80%, 67% and 53% respectively, three resistant strains of lactic acid bacteria had inhibition for Escherichia coli and Staphylococcus aureus in varying degrees; L1 had best inhibitory effect, the antibacterial diameter were 18.3mm and 16.4mm respectively. The results showed that L1 and L2 could considered to be candidate viable lactic acid bacteria strains resistant formulations.