火棘果不同极性部位提取物体外抗氧化研究

Study on antioxidant activity in vitro about different solvent extracts of fruits of Pyracantha fortuneana

目的:系统地评价火棘果粗分体系抗氧化活性,同时探究抗氧化能力与总多酚含量之间的关系。方法:用75%乙醇冷浸提取火棘果,分别用石油醚、乙酸乙酯、正丁醇和水分为四个不同极性部位,然后测定了各部位萃取物对DPPH自由基、ABTS+自由基的清除能力,并且测定了总还原力、FRAP值和总多酚含量,同时考察总酚含量与抗氧化活性的关系。结果:火棘果提取物的不同极性部位均有抗氧化活性,其中水、乙酸乙酯和正丁醇部位提取物表现出较好的抗氧化活性,石油醚部位的抗氧化活性最弱;各部位提取物的抗氧化活性与总多酚含量呈现较好的相关关系。其中,水部位提取物对DPPH和ABTS+自由基清除率最高,IC50值分别为(0.76±0.03)mg/m L和(1.71±0.10)mg/m L;乙酸乙酯部位FRAP值最大,为(382.20±4.72)μmol Fe2+/g干样;正丁醇部位总酚含量最高,为(2763±3.91)mg GAE/100g干样。

Objective： To evaluatethe antioxidant activity of fruits of Pyracantha fortuneana comprehensively, and explore the relationship between the antioxidant activity and total polyphenol content.Methods： Extracting fruits of Pyracantha fortuneana with 75% ethanol, petroleum ether, ethyl acetate, n- butanol and water extracts were obtained.All extracts were evaluated by DPPH free radical and ABTS＋ free radical scavenging activity, the total reducing power, the fluorescence recovery after photobleaching experiment （FRAP）and the total polyphenol content.Conclusions： All extracts of fruits of Pyracantha fortuneana exhibited antioxidant activities. The water, n-butanol and ethyl acetate extracts both exhibited preferable antioxidant activity,the petroleum ether extract had the weakest antioxidant activity.Antioxidant activities of both extracts were generally positively related with the total polyphenol content.The water extract showed the highest radical scavenging rate on DPPH and ABTS＋ ,the IC5o were （0.76±0.03） mg/mL and （1.71 ±0.10）mg/mL.The ethyl acetate extract showed the highest value of FRAP with（382.20 ±4.72）lamolFe2~/g.The highest content of total phenolics was from n-butanol extract with （2763 ± 3.91 ） mg GAE/100g.