苹果疏除幼果与成熟果多糖成分分析及抗氧化活性研究

Composition and antioxidant activity of polysaccharides from thinned- young- apple and matured- apple

为了探究苹果疏除幼果与成熟果多糖组成成分及抗氧化活性的的差异,采用热水浸提法从苹果幼果与成熟果果渣中分别提取多糖,通过PMP柱前衍生化高效液相色谱法(HPLC)分析两种多糖的单糖组成,并通过体外抗氧化评价体系对二者的抗氧化活性进行比较研究。结果表明:苹果疏除幼果与成熟果多糖均由10种单糖构成,但单糖比例存在较大差异,抗氧化结果表明:苹果疏除幼果与成熟果多糖均具有一定的还原能力,并对O-2·、DPPH·和·OH具有清除能力,但在相同浓度水平下,苹果疏除幼果多糖的抗氧化能力显著高于成熟果多糖。

The purpose of this study was to find out the difference of monosaccharide composition and the antioxidative activity of polysaccharides obtained from thinned-young-apple and matured-apple pomace. The conventional hot water methods were adopted to extract polysaccharides from thinned-young-apple and matured -apple pomace. High performance liquid chromatography（HPLC） based on precolumn derivatization with PMP was employed to analyze the monosaccharide composition of both polysaccharides.ln addition, the antioxidative activity of both polysaccharides were evaluated in vitro. The results indicated that both polysaccharides were composed of 10 kinds of monosaccharides, while the proportion of monosaccharides were different. The antioxidation experiments showed that two kinds of polysaccharides both had the reducing powers and the scavenging activity to ·OH, DPPH- and O-2 .But the antioxidative activities of thinned- young- apple polysaccharides were higher than matured-apple pomace polysaccharides under the same concentration level. Thus,both the monosaccharides composition and antioxidant activity of polysaccharides from thinned-young- apple and matured-apple were different.