3种丛枝菌根真菌对不同种源黄檗幼苗的影响

Effects of three Arbuscular Mycorrhizas on Different Provenances of Amur Cork Seedings

丛枝菌根(Arbuscular mycorrhizal,AM)作为分布最广泛的菌根类型,在植物生长和环境改善中起着非常重要的作用。许多研究也证实,丛枝菌根能够影响植物次生代谢过程,使次生代谢产物发生变化。本实验选用了3种不同的丛枝菌根真菌(Glomus spp.)进行接菌实验,同时选取了吉林、黑河和太空育种3个种源的黄檗幼苗作为研究对象。通过不同菌种和不同种源的结合,来探究丛枝菌根真菌对黄檗幼苗生长情况及生物碱含量的影响作用,以及这种影响作用在不同种源间的差异。本实验的结果显示,接种丛枝菌根真菌的黄檗幼苗生长情况及小檗碱、药根碱和掌叶防己碱3种生物碱含量都不同程度地优于对照组,说明丛枝菌根真菌在黄檗幼苗的生长和生物碱含量的积累方面均有一定的促进作用。在对生物碱含量的影响方面,对小檗碱和药根碱含量影响作用最显著的是摩西球囊霉,对掌叶防己碱含量的影响作用最显著的是根内球囊霉。同时,从实验结果我们还可以看出,本实验选取的3种丛枝菌根真菌对黄檗幼苗生物碱含量的影响作用的大小关系在不同种源间存在明显差异,说明丛枝菌根真菌对黄檗幼苗生物碱含量的影响受到黄檗种源的影响。

As the most widely distributed Mycorrhizal type, arbuscular mycorrhizal plays a very important role in plant growth and environmental improvement. Many studies confirmed that the arbuscular mycorrhizal can affect the process of plant secondary metabolism, and kinds of arbuscular mycorrhizal fungis, and chose three change the secondary metabolites. We screened three different sources of amur cork seedings as the research object in the inoculation experiment. By combining the different species and different kinds of sources, we explored the impact of the arbuscular mycorrhizal fungi on the growth and alkaloid content of amur cork and its difference among different provenances. The growth and the alkaloid content of amur cork with arbuscular mycorrhizal fnngis are better than those of the control group. The mycorrhizal fungi can effectively promote the growth of amur cork and the accumulation of alkaloid content. Overall, it is the Glomus mosseae that affects the content of berberine and jatrorrhizine mostly, and for palmatine it is Glomus intraradices. From the experimental results, there is difference among provenances in the impact on alkaloid with arbuscular mycorrhizal, and the difference may be related to the provenance.