对奶牛结核病平行检测的不同方法

Parallel Detection of Bovine Tuberculosis with Different Assays

[目的]综合分析奶牛的健康状况,评价不同检测方法的优缺点,提出实际应用建议。[方法 ]同时采用单纯牛结核菌素(PPD)皮内变态反应试验(TST,使用国产PPD简称国产TST、使用进口PPD简称进口TST)、欧盟比较变态反应试验(SICTT)和牛结核γ-干扰素酶联免疫吸附试验(IFN-γ-ELISA),对河北省唐山市某奶牛场存栏的115头牛进行结核病平行检测。[结果 ]经综合判定牛型结核阳性15头(其中5头为牛型、禽型双阳性)、阴性100头(其中1头为禽型阳性),牛型结核阳性率13.04%、禽型结核阳性率5.22%、二者混合感染率为4.35%。试验表明,对于伴有非结核分枝杆菌感染的牛群,仅靠一种活体检测方法难以做出牛结核病准确诊断。[结论 ]因此,可采用进口TST初筛,阳性和可疑牛用IFN-γ-ELISA复检以排除部分假阳性,对于早期感染牛场可直接采用IFN-γ-ELISA方法检测。

115 cattle in a dairy cattle farm in Tangshan,Hebei province were detected with pure bovine tuberculin （PPD）skin allergy test（TST,using domestic PPD as domestic TST,with imported PPD as imported TST）, single intradermal comparative cervical tuberculin test（SICCT）and gamma interferon enzyme-linked immune-sorbent assay（IFN-γ-ELISA）at the same time to analyze the cattle health status,evaluate the advantages and disadvantages of different assays and put forward applicable suggestions. The results showed there were 15 TB positive cattle（including 5 mixed infected with bovine and avian tuberculosis）,100 negative cattle（including 1 avian tuberculosis positive）. The TB positive rate was 13.04%,the avian tuberculosis positive rate was 5.22%,mixed infection rate was 4.35%. Tests showed that for cattle herd with non-mycobacterium tuberculosis infection ,it was dififcult to conifrm the bovine tuberculosis infection only by one test. Therefore,it was recommend ifrst to use the imported TST and then to retest the positive and suspicious ones by IFN-γ-ELISA to eliminate the false positives. For early infected herds,IFN-γ-ELISA test can be directly used for detection.