番红花多糖的提取及耐缺氧能力研究

Study on Extraction of Polysaccharides from Saffron and Anti-anoxia Ability

以番红花为原料,研究番红花多糖的响应面提取优化及耐缺氧能力。结果表明:番红花多糖提取最佳工艺条件为超声波功率为253.5 W,超声波时间为31.9 min,乙醇质量分数为83.9%,液料比值为16.4(mL/g)。同时,番红花多糖低剂量组、中剂量组和高剂量组与对照组比较,在常压缺氧条件下,小鼠存活时间分别延长了7.79%,10.9%和16.66%;在亚硝酸钠所致缺氧条件下,小鼠存活时间分别延长了20.71%,26.31%和37.06%,表明番红花多糖具有较好的耐缺氧能力。

The extraction technology and anti-anoxia ability of polysaccharides from saffron were mainly discussed. The optimum extraction condition of polysaccharides from saffron was determined, such as ultrasonic frequency for 253.5 W, ultrasonic extraction time for 31.9 min, ethanol concentration for 83.9%, and ratio of solution to material for 16.4（m L/g）. Meanwhile, compared with control group, survival time of low dose group, middle dose group and high dose group of polysaccharides from saffron were extended 7.79%, 10.9% and 16.66% under normal pressure hypoxia condition. They were extended 20.71%, 26.31% and 37.06% under condition caused by sodium nitrite anoxic, which showed polysaccharides from saffron had anti-anoxia ability.