ELISA法检测花生油中黄曲霉毒素B1前处理方法的改进研究

目的：对国标GB/T 5009.22-2003中第二法ELISA法测定花生油中黄曲霉毒素B1的样品前处理进行改良。方法：通过分别考察不同浓度的甲醇提取溶剂,分液漏斗萃取的不同振荡力度及频率,pH的调节对免疫反应的影响等。结果：提出了样品处理的最佳组合：使用60%甲醇提取液,分液漏斗萃取最优转速250rpm,振荡时间15min,样品提取物pH调节为6-8。新改进方法下样品加标回收率在80~110%之间。提高了ELISA法检测花生油黄曲霉毒素B1的效率和准确度。

objective：The ELISA detection method of Aflatoxin B1 in peanut oil should be optimized in national standard（GB/T 5009. 22--2003）.Method：Respectively inspect different concentration of methanol extraction solvent,and extract different oscilation intensity and frequency with separating funnel,as well as study the effect of pH adjustment on the immune response.Result：Abstract the best combination of sample processing by using 60% methanol extract,the optional way of separating funnel extraction speech is 250rpm,the oscillation time is 15min,pH adjustment of sample extracts is 6—8.The sample standard addition recovery rate is between 80% and 110% with the new improved method.It helps improve the ELISA’s efficiency and accuracy of detecting Aflatoxin B1 in peanut oil.