食品中人参总皂苷的测定方法研究

Determination of ginsenosides in foods

目的建立一种快速准确的分光光度法测定食品中人参皂苷的含量。方法采用不同前处理方式对不同类型的样品进行预处理,应用分光光度法测定人参皂苷的含量。结果人参皂苷在0μg^100μg范围内具有良好的线性,相关系数为0.9981,检出限为0.0025g/100g,相对标准偏差(RSD)为2.36%~8.56%,加标回收率在80.8%~103.3%之间。结论:该方法简便快速、准确灵敏,适用于测定食品中人参皂苷的含量。

Objective To establish a content Spectrophotometric method for rapid and accurate for the determination of ginsenosides in foods. Methods： Use of different pretreatment methods on different types of sample pretreatment, ginsenoside content determination by spectrophotometry. Results Ginsenoside in 0 μ g ～100 μ g with good linearity within the scope, the correlation coefficient was 0.9981 ,the detection limit of lead was 0.0025g/100g, the relative standard deviation （RSD） ranged from 2.36% to 8.56%, the recovery rate was 80.8% ～ 103.3% of standard addition. Conclusion The method is simple, rapid, accurate and sensitive, suitable for the content determination of Ginsenoside in foods.