真菌对自然杀伤细胞内干扰素-γ和白细胞介素-4表达的影响

Effect of fungus on expression of interferon-γ and interleukin-4 in natural killer cells

目的 探讨烟曲霉、白假丝酵母菌的不同菌体形态对脐血来源细胞因子诱导自然杀伤（NK）细胞内干扰素（IFN）-γ和白细胞介素（IL）-4表达的影响.方法 将NK细胞与真菌（烟曲霉孢子或菌丝、白假丝酵母菌孢子或菌丝）按10∶1的比例直接混合或隔离培养分为若干实验组,另设一个NK细胞空白对照组.培养6h后通过流式细胞仪检测各组NK细胞内IFN-γ和IL-4的表达.采用方差分析或SNK-q检验比较各组间IFN-γ和IL-4的表达.结果 空白对照组NK细胞内IFN-γ的表达率为（15.11±2.60）％,NK细胞与烟曲霉菌丝、白假丝酵母菌孢子和菌丝直接接触后细胞内IFN-γ的表达率分别为（20.12±0.53）％,（20.69±0.34）％,（20.8±0.37）％,间接接触后细胞内IFN-γ的表达率分别为（21.40±0.53）％,（20.57±1.09）％,（20.20 ±0.51）％,细胞内IFN-γ的表达较空白对照组显著升高（P均＜0.01）;而NK细胞与烟曲霉孢子直接、间接接触作用后细胞内IFN-γ的表达率分别为（14.33±0.98）％,（14.97±1.53）％,与对照组比较差异无统计学意义（P值均＞0.05）.空白对照组NK细胞内IL-4的表达率为（1.23±0.05）％,NK细胞与烟曲霉孢子和菌丝、白假丝酵母菌孢子和菌丝直接接触后细胞内IL-4的表达率分别为（1.25±0.06）％,（1.21±0.03）％,（1.22±0.46）％,（1.26±0.1 1）％,间接接触后细胞内IL-4的表达率分别为（1.21±0.06）％,（1.25±0.04）％,（1.27±0.03）％,（1.26±0.1）％,真菌刺激对NK细胞胞内IL-4的表达无显著影响（P值均＞0.05）.结论 真菌刺激可抑制NK细胞胞内IFN-γ的释放,但对IL-4的释放无显著影响.

Objective To investigate the effect of fungus Aspergillus fumigatus and Candida albicans on the expression of endocellular interferon-γ （IFN-γ） and interleukin-4 （IL-4） in cytokineinduced natural killer （NK） cells.Methods NK cells were cultured with Aspergillusfumigatus or Candida albicans by non-contact or direct-contact methods with a ratio of NK cells to fungus of 10 ∶ 1.The expressions of IFN-γ and IL-4 in NK cells were evaluated by flow cytometry after co-cultured for 6 h.Analysis of variance or SNK-q test was used to compare the expressions of IFN-γ and IL-4 among different groups.Results The IFN-γexpression rates in NK cells with direct contacting to Aspergillus fumigatus hyphae,or to different morphotypes of Candida albicans were （20.12 ± 0.53） ％,（20.69 ± 0.34） ％ and （20.8 ±0.37）％ respectively,while IFN-γexpression in NK cells with indirect contacting to fumigatus hyphae,or to different morphotypes of Candida albicans were （21.40 ± 0.53） ％,（20.57 ± 1.09） ％ and （20.20 ±0.51） ％ respectively,and all were significantly higher than that in the blank group [（15.11 ± 2.60） ％,all P ＞ 0.05].The IFN-γ expression rates in the Aspergillus fumigatus spores direct and indirect contacting groups were （14.33 ± 0.98） ％ and （14.97 ± 1.53） ％,which were not of significant difference compared with the blank group （P ＞ 0.05）.The IL-4 expression rates in NK cells with direct contacting to different morphotypes of Aspergillus fumigatus and Candida albicans were （1.25 ± 0.06） ％,（1.21 ± 0.03） ％,（1.22 ± 0.46） ％ and （1.26 ± 0.11） ％,while those in indirect contacting groups were （1.21 ± 0.06） ％,（1.25 ±0.04）％,（1.27 ±0.03）％ and （1.26 ±0.1）％,which were not of significant difference compared with the blank group [（1.23 ± 0.05） ％,all P ＞ 0.05].Conclusion Fungus stimuli can reduce the secretion of IFN-γ in NK cells,but have not significant influence on the secretion of IL-4.