副溶血弧菌在玻璃器皿上的粘附及去除

The Remaining Vibrio parahaemolyticus on Glassware and Removal Technology

[目的]研究副溶血性弧菌在玻璃器皿上的粘附及去除技术。[方法]以载玻片模拟厨房中的玻璃类和陶瓷类厨具,选用酒精、醋酸及乳酸链球菌素作为抑菌剂对人工污染粘附到载玻片上的副溶血性弧菌进行处理,通过观察副溶血性弧菌在载玻片上的存活数和去除率考察3种抑菌剂及其协同抑菌作用。[结果]载玻片上粘附的副溶血性弧菌的活菌数经生理盐水和不同浓度的酒精、醋酸和乳酸菌链球菌素作用后均有一定程度的降低,抑菌剂组残留的活菌数(残留率)明显低于生理盐水对照组,各抑菌组的抑菌能力均随着抑菌剂浓度的增加而增强。协同抑菌试验结果表明,p H 4.0、35%酒精、1.0 g/L乳酸链球菌素的组合能有效抑制副溶血性弧菌在玻片上的粘附,具有良好的协同效应。[结论]副溶血性弧菌在玻璃器皿上有一定的粘附力,但酒精、醋酸和乳酸菌链球菌素具有较好的除菌效果。

[ Objective] To study the adhesion and degerming of Vibrio parahaemolyticus on glassware. [ Method] The effects of three naturaledible components including acetic acid, alcohol and Nisin on the survival rate of Vibrio parahaemolyticus on glassware were explored throughsingle factor and orthogonal array experiments. [ Result] It was found that bacterial population on glassware was reduced when Vibrio parahae-molyticus ceils were treated with acetic acid, alcohol and Nisin, and the residual rate is lower than the normal saline control group obviously.The orthogonal array optimization showed that the combined treatment of acetic acid, alcohol and Nisin could result in a synergistic inhibitoryeffect on Vibrio parahaemolyticus. Vibrio parahaemolyticus could be effectively inhibited after treatment with acetic acid solution（ pH 4.0） con-taining 35% alcohol and 1.0 g/L Nisin. [ Conclusion] These results suggest that Vibrio parahaemolyticus can adhere to the glassware, and thecombined use of acetic acid, alcohol and Nisin can offer a compound antibacterial agent for reducing the risk of Vibrio paraaelyticus infec-tion during seafood consumption.