高效液相色谱法测定抗风湿液中染料木苷、染料木素、去氢二异丁香酚、五味子甲素的含量

HPLC determination of genistin, genistein, dehydrodiisoeugenol and deoxyschizandrin in Kangfengshi liquid

目的采用多波长HPLC梯度洗脱法建立同时测定抗风湿液中染料木苷、染料木素、去氢二异丁香酚和五味子甲素含量的方法。方法采用C18色谱柱（250 mm×4.6 mm,5μm）;流速：1.0 mL·min-1;流动相A为乙腈,流动相B为0.5%醋酸溶液,梯度洗脱;检测波长：染料木苷和染料木素为263 nm,去氢二异丁香酚为274 nm,五味子甲素为250 nm;柱温为35℃。结果染料木苷、染料木素、去氢二异丁香酚和五味子甲素质量浓度分别在9.65～193.00μg·mL-1（r=0.999 9）、7.25～145.00μg·mL-1（r=0.999 7）、5.90～118.00μg·mL-1（r=0.999 2）、5.30～106.00μg·mL-1（r=0.999 6）时与其峰面积呈良好的线性关系;染料木苷、染料木素、去氢二异丁香酚和五味子甲素的平均加样回收率分别为98.8%、99.1%、96.9%、98.1%,RSD分别为1.0%、1.3%、1.3%、1.5%（n=6）。结论该方法是一种快速、灵敏、准确的分析方法,可作为抗风湿液的含量控制方法。

Objective To develop an HPLC method for the simultaneous determination of genistin, genistein, dehydrodii- soeugenol and deoxyschizandrin in Kangfengshi liquid. Methods The chromatograhic separation was performed on C18 column （250 mm×4.6 mm, 5 μm）, a linear elution of acetonitrile and 0.5% acetic acid solution was adopted at the flow rate of 1.0 mL ·min -1. The detection wavelength were 263 nm for genistin and genistein, 274 nm for dehydrodiisoeu- genol, 250 nm for deoxyschizandrin. The column temperature was 35 ℃. Results Genistin, genistein, dehydrodiisoeuge- nol and deoxyschizandrin had a good linearity at 9.65 - 193.00μg · mL - 1 （r = 0.999 9）, 7.25 - 145.00 μg · mL -1 （r = 0.999 7）, 5.90 - 118.00 μg · mL- l（r = 0.999 2）, 5.30 -106.00 μg · mL- 1（r = 0.999 6）, respectively. The aver- age recoveries and RSDs were 98.8% （1.0%）, 99.1% （1.3%）, 96.9% （1.3%） and 98.1% （1.5%）, respectively. Conclu- sion The method is simple, convenient and may be used for the determination of genistin, genistein, dehydrodiisoeu- genol and deoxyschizandrin in Kangfengshi liquid.