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高效液相色谱法测定氨碘肽滴眼液中5种氨基酸的含量 被引量:2

HPLC determination of 5 amino acid of Amiotide eye drops
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摘要 目的采用HPLC法测定氨碘肽滴眼液中丝氨酸(Ser)、甘氨酸(Gly)、组氨酸(His)、异亮氨酸(Ile)、亮氨酸(Leu)的含量。方法采用Ocean-C18色谱柱(250 mm×4.6 mm,5μm);流动相A:乙腈-水(4∶1),流动相B:0.1 mol·L-1乙酸钠-乙腈(97:3);流速为1.0 mL·min-1;柱温:25℃;检测波长:254 nm;用异硫氰酸苯酯(PITC)作为柱前衍生化试剂,采用柱前衍生HPLC法进行分析。结果各种氨基酸能良好地分离,衍生化试剂对氨基酸分析无干扰;平均回收率在98%~102%,RSD均〈3%。结论该方法简便、准确、可靠,可用于氨碘肽滴眼液中氨基酸含量的检测。 Objective To establish a HPLC method to determine the 5 amino acid in Amiotide eye drops. Methods A Ocean-C18 (250 mm × 4.6 mm, 5μm) column was used. The mobile phase A was acetonitrile-water (4 : 1) and B was 0.1 mol · L -1 sodium acetate-acetonitrile (97 : 3). The flow rate was 1.0 mL · min -1. The column temperature was 25 ℃ . The detection wavelength was 254 nm. Phenylisothiocyanate was used as the derivatizing agent. Results Complete separation was achieved among 5 amino acid under optimun conditions. No interference from derivative reagent was found. The average recoveries were at 98% - 102%, and RSDs were less than 3%. Conclusion The method is simple, accurate, reliable and suitable for the quality control of Amiotide eye drops.
出处 《中南药学》 CAS 2014年第9期909-912,共4页 Central South Pharmacy
基金 国家自然科研基金--青年基金项目(No.81100654)
关键词 氨碘肽滴眼液 异硫氰酸苯酯 柱前衍生化 高效液相色谱法 氨基酸 Amiotide eye drop phenylisothiocyanate pre-column derivatization HPLC amino acid
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